Genotypic Analysis of the 5′‐Untranslated Region of a Pestivirus Strain Isolated from Human Leucocytes

Microbiology and Immunology - Tập 41 Số 10 - Trang 829-834 - 1997
Massimo Giangaspero1, Ryô Harasawa2, A. Verhulst3
1Special Pathology and Veterinary Medical Clinic Institute, Faculty of Veterinary Medicine The University of Milan Via Celoria 1–20133 Milan Italy
2Animal Center for Biomedical Research, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113 Japan
3Prince Leopold Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium

Tóm tắt

AbstractThe 5′‐untranslated genomic region of the pestivirus strain Europa, originated in human leucocytes and previously identified as bovine diarrhea virus (BVDV), was amplified by reverse transcription‐PCR and sequenced. Analyses based on primary nucleotide sequence homology and on secondary palindromic sequence structures characteristic to genotypes revealed that this human isolate should be assigned to a novel genotype of pestivirus, type Ic. This newly emerged genotype was related to, but distinguishable from the three known BVDV genotypes, Ia, Ib and II. Three other bovine field isolates of BVDV originated from Germany were also found to belong to this new genotype Ic. Within pestivirus genotype Ic strains, the overall nucleotide sequence homology was 95–96%, and 88–92%, 88–90% and 77–79% with the other BVDV genotypes Ia, Ib and II, respectively. With the strains from border disease virus (genotype III) and hog cholera virus (genotype IV), homologies were less than 75%.

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Microbiology and Immunology

Tập 41 Số 10

829-834

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