Genomic characterization of human rotavirus G8 strains from the African rotavirus network: Relationship to animal rotaviruses

Journal of Medical Virology - Tập 81 Số 5 - Trang 937-951 - 2009
Mathew D. Esona1,2, A. Geyer3,2, Nicola Page2,4, Abdelhalim Trabelsi5,2, I. Fodha5,2, Maryam Aminu6,2, V.A. Agbaya7,2, Tsion Bizuneh8,2, Tara Kerin1, George Armah2,9, A. Duncan Steele2,10, Roger I. Glass11, Jon R. Gentsch1
1Gastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, NCIRD, CDC, Atlanta, Georgia
2Members of the African Rotavirus Network.
3MRC/DPRU, University of Limpopo, Pretoria campus, South Africa
4Viral Gastroenteritis Unit, National Institute for Communicable Disease, Sandringham, South Africa
5Laboratory of Bacteriology-Virology, University Hospital Sahloul, Sousse, Tunisia
6Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria
7Laboratoire de Bacteriologie/Virologie, Institut Pasteur de Cote-d'Ivoire/DVE, Abidjan, Cote-d'Ivoire
8Ethiopian Institute of Virology, Addis Ababa, Ethiopia
9Noguchi Memorial Research Institute Accra, Ghana
10PATH, NW, Seattle, Washington
11Fogarty International Center, National Institutes of Health, Bethesda, Maryland

Tóm tắt

Abstract

Global rotavirus surveillance has led to the detection of many unusual human rotavirus (HRV) genotypes. During 1996–2004 surveillance within the African Rotavirus Network (ARN), six P[8],G8 and two P[6],G8 human rotavirus strains were identified. Gene fragments (RT‐PCR amplicons) of all 11‐gene segments of these G8 strains were sequenced in order to elucidate their genetic and evolutionary relationships. Phylogenetic and sequence analyses of each gene segment revealed high similarities (88–100% nt and 91–100% aa) for all segments except for gene 4 encoding VP4 proteins P[8] and P[6]. For most strains, almost all of the genes of the ARN strains other than neutralizing antigens are related to typical human strains of Wa genogroup. The VP7, NSP2, and NSP5 genes were closely related to cognate genes of animal strains (83–99% and 97–99% aa identity). This study suggests that the ARN G8 strains might have arisen through VP7 or VP4 gene reassortment events since most of the other gene segments resemble those of common human rotaviruses. However, VP7, NSP2 (likely), and NSP5 (likely) genes are derived potentially from animals consistent with a zoonotic introduction. Although these findings help elucidate rotavirus evolution, sequence studies of cognate animal rotavirus genes are needed to conclusively determine the specific origin of those genes relative to both human and animal rotavirus strains. J. Med. Virol. 81:937–951, 2009. © 2009 Wiley‐Liss, Inc.

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Journal of Medical Virology

Tập 81 Số 5

937-951

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