Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae

BMC Microbiology - Tập 23 - Trang 1-12 - 2023
Qian Li1,2, Peng Feng1,3, Hao Tang4, Fujia Lu1, Borui Mou1, Lan Zhao5, Nan Li1, Yaojun Yang1,3, Chun Fu1,3, Wencong Long1,3, Ximeng Xiao1,3, Chaohao Li6, Wei Wu6, Gang Wang1,7, Beidong Liu8,9, Tianle Tang10, Menggen Ma2, Hanyu Wang1,3
1College of Life Science, Leshan Normal University, Leshan, China
2Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, People’s Republic of China
3Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, College of Life Science, Leshan Normal University, Leshan, China
4Key Laboratory of Land Resources Evaluation and Monitoring in Southwest, Ministry of Education, Sichuan Normal University, Chengdu, China
5Jiangxi Forestry Science and Technology Promotion and Publicity Education Center, Nanchang, China
6Leshan Institute of Product Quality Supervision and Testing, Leshan, China
7Engineering Research Center of Sichuan Province Higher School of Local Chicken Breeds Industrialization in Southern Sichuan, College of Life Science, Leshan Normal University, Leshan, China
8Department of Chemistry and Molecular Biology, University of Gothenburg, Göteburg, Sweden
9State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China
10Key Laboratory of Tropical Transitional Medicine of Ministry of Education, Hainan Medical University, Haikou, China

Tóm tắt

In bioethanol production, the main by-product, 5-hydroxymethylfurfural (HMF), significantly hinders microbial fermentation. Therefore, it is crucial to explore genes related to HMF tolerance in Saccharomyces cerevisiae for enhancing the tolerance of ethanol fermentation strains. A comprehensive analysis was conducted using genome-wide deletion library scanning and SGAtools, resulting in the identification of 294 genes associated with HMF tolerance in S. cerevisiae. Further KEGG and GO enrichment analysis revealed the involvement of genes OCA1 and SIW14 in the protein phosphorylation pathway, underscoring their role in HMF tolerance. Spot test validation and subcellular structure observation demonstrated that, following a 3-h treatment with 60 mM HMF, the SIW14 gene knockout strain exhibited a 12.68% increase in cells with abnormal endoplasmic reticulum (ER) and a 22.41% increase in the accumulation of reactive oxygen species compared to the BY4741 strain. These findings indicate that the SIW14 gene contributes to the protection of the ER structure within the cell and facilitates the clearance of reactive oxygen species, thereby confirming its significance as a key gene for HMF tolerance in S. cerevisiae.

Tài liệu tham khảo

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