Đặc điểm biểu hiện toàn bộ hệ gen của việc tăng cường ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) trong phản ứng với cytokinin

Springer Science and Business Media LLC - Tập 277 - Trang 115-137 - 2006
Dong Ju Lee1, Jin-Young Park1, Su-Jin Ku1, Young-Min Ha1, Sunmi Kim1, Myung Duk Kim1, Man-Ho Oh1, Jungmook Kim1
1Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, South Korea

Tóm tắt

Cá thể loại-A ARRs trong hệ thống tín hiệu hai thành phần cytokinin hoạt động như các yếu tố điều hòa tiêu cực đối với tín hiệu cytokinin ngoại trừ ARR4, tuy nhiên cơ chế phân tử mà các ARRs loại A điều chỉnh tín hiệu cytokinin vẫn chưa rõ ràng. Để hiểu biết sâu hơn về chức năng phân tử của ARR loại A trong phản ứng với cytokinin, chúng tôi đã tìm kiếm các thành phần hoạt động hạ lưu của protein ARR loại A bằng cách điều tra ảnh hưởng của việc tăng cường biểu hiện ARR7 lên sự biểu hiện gen được điều chỉnh bởi cytokinin với việc phân tích toàn bộ hệ gen Affymetrix. Để khảo sát phản ứng sớm với cytokinin, cây trồng đã được điều trị với cytokinin trong 30 phút hoặc 2 giờ, sau đó tiến hành phân tích GeneChip. Phân tích phân cụm phân cấp dữ liệu GeneChip của chúng tôi cho thấy việc tăng cường biểu hiện ARR7 có tác động ức chế rõ rệt đối với nhiều nhóm gen được điều chỉnh bởi cytokinin. Cụ thể, sự kích thích của tất cả các ARRs loại A ngoại trừ ARR22, và AHK(ARABIDOPSIS HISTIDINE KINASE)1 và AHK4 đã bị ức chế bởi ARR7. Việc biểu hiện gene expansin bị kích thích bởi cytokinin của hầu hết 12 gene expansin đã bị ARR7 ức chế, cho thấy sự tham gia tiềm năng của ARR7 trong mở rộng tế bào và phát triển cây. Việc điều chỉnh tăng 5 gene cytokinin oxidase bởi cytokinin đã bị ARR7 tác động tiêu cực. Phân tích GeneChip của chúng tôi gợi ý rằng ARR7 chủ yếu hoạt động như một yếu tố ức chế phiên mã cho nhiều gen được điều chỉnh sớm bởi cytokinin mã hóa các yếu tố phiên mã, truyền tín hiệu, phát triển cây trồng và trao đổi chất tế bào, điều này có thể là nguyên nhân dẫn đến giảm độ nhạy của các cây trồng chuyển gen Arabidopsis tăng cường biểu hiện ARR7 với các cytokinin ngoại sinh.

Từ khóa

#ARR7 #cytokinin #điều chỉnh gen #biểu hiện cây trồng #phân tử sinh học

Tài liệu tham khảo

Achard P, Vriezen WH, Van Der Straeten D, Harberd NP (2003) Ethylene regulates Arabidopsis development via the modulation of DELLA protein growth repressor function. Plant Cell 15:2816–2825 Bilyeu KD, Cole JL, Laskey JG, Riekhof WR, Esparza TJ, Kramer MD, Morris RO (2001) Molecular and biochemical characterization of a cytokinin oxidase from maize. Plant Physiol 125:378–386 Bortiri E, Chuck G, Vollbrecht E, Rocheford T, Martienssen R, Hake S (2006) ramosa2 encodes a LATERAL ORGAN BOUNDARY domain protein that determines the fate of stem cells in branch meristems of maize. Plant Cell 18:574–585 Brenner WG, Romanov GA, Köllmer I, Bürkle L, Schmülling T (2005) Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades. Plant J 44:314–333 Brugière N, Jiao S, Hantke S, Zinselmeier C, Roessler JA, Niu X, Jones RJ, Habben J (2003) Cytokinin oxidase gene expression in maize is localized to the vasculature, and is induced by cytokinins, abscisic acid, and abiotic stress. Plant Physiol 132:1228–1240 Cantero A, Barthakur S, Bushart TJ, Chou S, Morgan RO, Fernandez MP, Clark GB, Roux SJ (2006) Expression profiling of the Arabidopsis annexin gene family during germination, de-etiolation and abiotic stress. Plant Physiol Biochem 44:13–24 Chatfield JM, Armstrong DJ (1986) Regulation of cytokinin oxidase activity in callus tissues of Phaseolus vulgaris L. cv Great Northern. Plant Physiol 80:493–499 Clough SJ, Bent AJ (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743 Cosgrove DJ, Li LC, Cho HT, Hoffmann-Benning S, Moore RC, Blecker D (2002) The growing world of expansins. Plant Cell Physiol 43:1436–1444 D’Agostino IB, Seruère J, Kieber JJ (2000) Characterization of the response of the Arabidopsis response regulator gene family to cytokinin. Plant Physiol 124:1706–1717 Davies PJ (ed) (2004) Plant hormones biosynthesis, signal transduction, action. Kluwer Academic Publishers, The Netherlands Downes BP, Crowell DN (1998) Cytokinin regulates the expression of a soybean β-expansin gene by a post-transcriptional mechanism. Plant Mol Biol 37:437–444 Ferreira FJ, Kieber JJ (2005) Cytokinin signaling. Curr Opin Plant Biol 8:518–525 Fu X, Harberd NP (2003) Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421:740–743 Gazzarrini S, McCourt P (2003) Cross-talk in plant hormone signalling: what Arabidopsis mutants are telling us. Ann Bot (Lond) 91:605–612 Gilmour SJ, Sebolt AM, Salazar MP, Everard JD, Thomashow MF (2000) Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation. Plant Physiol 124:1854–1865 Greenboim-Wainberg Y, Maymon I, Borochov R, Alvarez J, Plszewski N, Ori N, Eshed Y, Weiss D (2005) Cross talk between gibberellin and cytokinin: the Arabidopsis GA response inhibitor SPINDLY plays a positive role in cytokinin signaling. Plant Cell 17:92–102 Hass C, Lohrmann J, Albrecht V, Sweere U, Hummel F, Yoo SD, Hwang I, Zhu T, Schäfer E, Kudla J, Harter K (2004) The response regulator 2 mediates ethylene signaling and hormone signal integration in Arabidopsis. EMBO J 12:3290–3302 Higuchi M, Pischke MS, Mähönen AP, Miyawaki K, Hashimoto Y, Seki M, Kobayashi M, Shinozaki K, Kato T, Tabata S, Helariutta Y, Sussman MR, Kakimoto T (2004) In planta functions of the Arabidopsis cytokinin receptor family. Proc Natl Acad Sci USA 8:8821–8826 Houba-Hérin N, Pethe C, d’Alayer J, Laloue M (1999) Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts. Plant J 17:615–626 Hutchison CE, Kieber JJ (2002) Cytokinin signaling in Arabidopsis. Plant Cell 14:S47–S59 Hwang I, Sheen J (2001) Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature 413:383–389 Hwang I, Chen HC, Sheen J (2002) Two-component signal transduction pathways in Arabidopsis. Plant Physiol 129:500–515 Imamura A, Kiba T, Tajima Y, Yamashino T, Mizuno T (2003) In vivo and in vitro characterization of the ARR11 response regulator implicated in the His-to-Asp phosphorelay signal transduction in Arabidopsis thaliana. Plant Cell Physiol 413:122–131 Inoue T, Higuchi M, Hashimoto Y, Seki M, Kobayashi M, Kato T, Tabata S, Shinozaki K, Kakimoto T (2001) Identification of CRE1 as a cytokinin receptor from Arabidopsis. Nature 409:1060–1063 Inukai Y, Sakamoto T, Ueguchi-Tanaka M, Shibata Y, Gomi K, Umemura I, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M (2005) Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling. Plant Cell 17:1387–1396 Iwakawa H, Ueno Y, Semiarti E, Onouchi H, Kojima S, Hirokazu T, Hasebe M, Soma T, Ikezaki M, Machida C, Machida Y (2002) The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat leaf lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper. Plant Cell Physiol 43:467–478 Kakimoto T (1996) CKI1, a histidine kinase homolog implicated in cytokinin signal transduction. Science 274:982–985 Kasuga M, Liu Q, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1999) Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotech 17:287–291 Kende H, Bradford K, Brummell D, Cho HT, Cosgrove D, Fleming A, Gehring C, Lee Y, McQueen-Mason S, Rose J, Voesenek LA. (2004) Nomenclature for members of the expansin superfamily of genes and proteins. Plant Mol Biol 55:311–314 Kiba T, Yamada H, Sato S, Kato T, Tabata S, Yamashino T, Mizuno T (2003) The type-A response regulator, ARR15, acts as a negative regulator in the cytokinin-mediated signal transduction in Arabidopsis thaliana. Plant Cell Physiol 44:868–874 Kiba T, Aoki K, Sakakibara H, Mizuno T (2004) Arabidopsis response regulator, ARR22, ectopic expression of which results in phenotypes similar to the wol cytokinin-receptor mutant. Plant Cell Physiol 45:1063–1077 Kiba T, Naitou T, Koizumi N, Yamashino T, Sakakibara H, Mizuno T (2005) Combinatorial microarray analysis revealing Arabidopsis genes implicated in cytokinin responses through the His→Asp phosphorelay circuitry. Plant Cell Physiol 46:339–355 Kim HJ, Kim YK, Park JY, Kim J (2002) Light signaling mediated by phytochrome plays an important role in cold-induced gene expression through the C-repeat/dehydration responsive element (C/DRE) in Arabidopsis thaliana. Plant J 29:693–704 Kim HJ, Ryu H, Hong SH, Woo HR, Lim PO, Lee IC, Sheen J, Nam HG, Hwang I (2006) Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis. Proc Natl Acad Sci USA 103:814–819 Kubo M, Kakimoto T (2000) The CYTOKININ-HYPERSENSITIVE genes of Arabidopsis negatively regulate the cytokinin-signaling pathway for cell division and chloroplast development. Plant J 23:385–394 Lee S, Lee EJ, Yang EJ, Lee JE, Park AR, Song WH, Park OK (2004) Proteomic identification of annexins, calcium-dependent membrane binding proteins that mediate osmotic stress and abscisic acid signal transduction in Arabidopsis. Plant Cell 16:1378–1391 Leibfried A, To JPC, Busch W, Stehling S, Kehle A, Demar M, Kieber JJ, Lohmann JU (2005) WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators. Nature 438:22–29 Lemaire SD (2004) The glutaredoxin family in oxygenic photosynthetic organisms. Photosynth Res 79:305–318 Lin W, Shuai B, Springer PS (2003) The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and adaxial-abaxial patterning. Plant Cell 15:2242–2252 Liu H, Wang S, Yu X, Yu J, He X, Zhang S, Shou H, Wu P (2005) ARL1, a LOB-domain protein required for adventitious root formation in rice. Plant J 43:47–56 Lohrmann J, Harter K (2002) Plant two-component signaling systems and the role of response regulators. Plant Physiol 128:363–369 Morris RO, Bilyeu KD, Laskey JG, Cheikh NN (1999) Isolation of a gene encoding a glycosylated cytokinin oxidase from maize. Biochem Biophys Res Commun 255:328–333 Nemhauser JL, Mockler TC, Chory J (2004) Interdependency of brassinosteroid and auxin signaling in Arabidopsis. PLOS Biol 2:1460–1471 Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C (2004) Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis. Plant Cell 16:1365–1377 Okushima Y, Overvoorde PJ, Arima K, Alonso JM, Chan A, Chang C, Ecker JR, Hughes B, Lui A, Nguyen D, Onodera C, Quach H, Smith A, Yu G, Theologis A (2005) Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19. Plant Cell 17:444–463 Osakabe Y, Miyata S, Urao T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2002) Overexpression of Arabidopsis response regulators, ARR4/ATRR1/IBC7 and ARR8/ATRR3, alters cytokinin responses differentially in the shoot and in callus formation. Biochem Biophys Res Commun 293:806–815 Park JY, Kim HJ, Kim J (2002) Mutation in domain II of IAA1 confers diverse auxin-related phenotypes and represses auxin-activated expression of Aux/IAA genes in steroid regulator-inducible system. Plant J 32:669–683 Parry G, Estelle M (2006) Auxin receptors: a new role for F-box proteins. Curr Opin Cell Biol 18:152–156 Pischke MS, Jones LG, Otsuga D, Fernandez DE, Drews GN, Sussman MR (2002) An Arabidopsis histidine kinase is essential for megagametogenesis. Proc Natl Acad Sci USA 99:15800–15805 Rashotte AM, Carson SDB, To JPC, Kieber J (2003) Expression profiling of cytokinin action in Arabidopsis. Plant Physiol 132:1988–2011 Rashotte AM, Mason MG, Hutchison CE, Ferreira FG, Schaller GE (2006) A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway. Proc Natl Acad Sci USA 103:11081–11085 Reed JW (2001) Roles and activities of Aux/IAA proteins in Arabidopsis. Trends Plant Sci 6:420–425 Sakai H, Aoyama T, Oka A (2000) Arabidopsis ARR1 and ARR2 response regulators operate as transcriptional activators. Plant J 24:703–711 Sakai H, Honma T, Aoyama T, Sato S, Kato T, Tabata S, Oka A (2001) ARR1, a transcription factor for genes immediately responsive to cytokinins. Science 294:1519–1521 Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y (2001) The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves. Development 128:1771–1783 Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410–417 Shuai B, Reynaga-Peña CG, Springer PS (2002) The LATERAL ORGAN BOUNDARIES gene defines a novel, plant-specific gene family. Plant Physiol 129:747–761 Suzuki T, Miwa K, Ishikawa K, Yamada H, Aiba H, Mizuno T (2001) Arabidopsis sensor His-kinase, AHK4, can respond to cytokinins. Plant Cell Physiol 42:107–113 Sweere U, Eichenberg K, Lohrmann J, Mira-Rodado V, Bäurle I, Kudla J, Nagy F, Schäfer E, Harter K (2001) Interaction of the response regulator ARR4 with phytochrome B in modulating red light signaling. Science 294:1108–1111 Tajima Y, Imamura A, Kiba T, Amano Y, Yamashino T, Mizuno T (2004) Comparative studies on the type-B response regulators revealing their distinctive properties in the His-to-Asp phosphorelay signal transduction of Arabidopsis thaliana. Plant Cell Physiol 45:28–39 Terrine C, Laloue M (1980) Kinetics of N6 − (Δ2 − isopentenyl)adenosine degradation in tobacco cells. Plant Physiol 65:1090–1095 To JPC, Haberer G, Ferreira FJ, Deruère J, Mason MG, Schaller GE, Alonso JM, Ecker JR, Kieber JJ (2004) Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. Plant Cell 16:658–671 Ueguchi C, Koizumi H, Suzuki T, Mizuno T (2001a) Novel family of sensor histidine kinase genes in Arabidopsis thaliana. Plant Cell Physiol 42:231–235 Ueguchi C, Sato S, Kato T, Tabata S (2001b) The AHK4 gene involved in the cytokinin-signaling pathway as a direct receptor molecule in Arabidopsis thaliana. Plant Cell Physiol 42:751–755 Urao T, Yakubov B, Satoh R, Yamaguchi-Shinozaki K, Seki M, Hirayama T, Shinozaki K (1999) A transmembrane hybrid-type histidine kinase in Arabidopsis functions as an osmosensor. Plant Cell 11:1743–1754 Vernon LP (1960) Spectrophotometric determination of chlorophyll and pheophytins in plant extracts. Anal Chem 32:1142–1150 White PJ, Bowen HC, Demidchik V, Nichols C, Davies JM (2002) Genes for calcium-permeable channels in the plasma membrane of plant root cells. Biochim Biophys Acta 1564:299–309 Xing S, Mario G, Zachgo S (2005) ROXY1, a member of the plant glutaredoxin family, required for petal development in Arabidopsis thaliana. Development 132:1555–1565 Yamada H, Suzuki T, Terada K, Takei K, Ishikawa K, Kumiko M, Yamashino T, Mizuno T (2001) The Arabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane. Plant Cell Physiol 42:1017–1023