Genome‐wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms

Protein Science - Tập 7 Số 4 - Trang 1029-1038 - 1998
Erik Wallin1, Gunnar von Heijne1
1Department of Biochemistry, Stockholm University, S-106 91 Stockholm, Sweden

Tóm tắt

Abstract

We have carried out detailed statistical analyses of integral membrane proteins of the helix‐bundle class from eubacterial, archaean, and eukaryotic organisms for which genome‐wide sequence data are available. Twenty to 30% of all ORFs are predicted to encode membrane proteins, with the larger genomes containing a higher fraction than the smaller ones. Although there is a general tendency that proteins with a smaller number of transmembrane segments are more prevalent than those with many, uni‐cellular organisms appear to prefer proteins with 6 and 12 transmembrane segments, whereas Caenorhabditis elegansandHomo sapienshave a slight preference for proteins with seven transmembrane segments. In all organisms, there is a tendency that membrane proteins either have manytransmembrane segments with short connecting loops or few transmembrane segments with large extra‐membraneous domains. Membrane proteins from all organisms studied, except possibly the archaeon Methanococcus jannaschii, follow the so‐called “positive‐inside” rule; i.e., they tend to have a higher frequency of positively charged residues in cytoplasmic than in extra‐cytoplasmic segments.

Từ khóa


Tài liệu tham khảo

10.1002/j.1460-2075.1987.tb02670.x

10.1002/(SICI)1097-0134(199708)28:4<465::AID-PROT1>3.0.CO;2-9

10.1126/science.278.5346.2123

10.1093/emboj/17.20.6061

10.1126/science.273.5278.1058

Claros MG, 1994, TopPred II: An improved software for membrane protein structure prediction, CABIOS, 10, 685

10.1126/science.8178151

10.1093/protein/10.6.673

10.1016/S0014-5793(97)00402-X

10.1002/j.1460-2075.1995.tb00321.x

10.1146/annurev.bb.15.060186.001541

10.1126/science.7542800

10.1126/science.270.5235.397

10.1074/jbc.272.10.6119

10.1016/0014-5793(91)80440-E

10.1111/j.1432-1033.1992.tb16892.x

10.1006/jmbi.1997.1412

Goffeau A, 1997, The yeast genome directory, Nature (Suppl), 387, 1

10.1073/pnas.86.15.5786

10.1093/nar/24.22.4420

10.1002/pro.5560010313

10.1038/376660a0

10.1021/bi00176a037

10.1093/dnares/3.3.109

10.1093/emboj/16.9.2197

10.1038/37052

10.1038/36786

10.1002/j.1460-2075.1995.tb06994.x

10.1093/protein/10.1.1

10.1016/0092-8674(90)90390-Z

Persson B, 1996, Topology prediction of membrane proteins, Protein Sci, 5, 363, 10.1002/pro.5560050221

10.1023/A:1026353225758

10.1146/annurev.bi.65.070196.001415

10.1002/pro.5560050824

10.1111/j.1432-1033.1993.tb17885.x

10.1016/0014-5793(95)00551-J

10.1093/nar/22.22.4673

10.1038/41483

10.1126/science.272.5265.1136

10.1002/j.1460-2075.1986.tb04601.x

10.1016/0022-2836(92)90934-C

10.1016/S0079-6107(97)85627-1

10.1111/j.1432-1033.1988.tb14150.x

10.1083/jcb.137.3.555

10.1093/protein/8.7.693

10.1074/jbc.271.41.25590

Thông tin
Thông tin xuất bản

Protein Science

Tập 7 Số 4

1029-1038

Thông tin tác giả