Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana

American Association for the Advancement of Science (AAAS) - Tập 301 Số 5633 - Trang 653-657 - 2003
José M. Alonso1,2,3, Anna N. Stepanova1,2,3, Thomas J. Leisse1,2,3, Christopher J. Kim1,2,3, Huaming Chen1,2,3, Paul Shinn1,2,3, Denise K. Stevenson1,2,3, Justin Zimmerman1,2,3, Pascual Barajas1,2,3, Rosa Cheuk1,2,3, Carmelita Gadrinab1,2,3, Collen Heller1,2,3, Albert Jeske1,2,3, Eric Koesema1,2,3, Cristina C. Meyers1,2,3, Holly Parker1,2,3, Lance Prednis1,2,3, Yasser Ansari1,2,3, Nathan Choy1,2,3, Hashim Deen1,2,3, M. Geralt1,2,3, Nisha Hazari1,2,3, Emily Wu1,2,3, Meagan Karnes1,2,3, Celene B. Mulholland1,2,3, Ral Ndubaku1,2,3, Ian Schmidt1,2,3, Plinio Guzmán1,2,3, Laura Aguilar‐Henonin1,2,3, Markus Schmid1,2,3, Detlef Weigel1,2,3, David E. Carter1,2,3, Trudy Marchand1,2,3, Eddy Risseeuw1,2,3, Debra Brogden1,2,3, Albana Zeko1,2,3, William L. Crosby1,2,3, Charles C. Berry1,2,3, Joseph R. Ecker1,2,3
1Department of Family/Preventive Medicine, University of California, San Diego, CA 92093, USA.
2Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
3National Research Council Plant Biotechnology Institute, Saskatoon, SK, S7N 0W9 Canada.

Tóm tắt

Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the ∼29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.

Từ khóa


Tài liệu tham khảo

10.1038/35048692

10.1038/46558

10.1126/science.285.5429.901

10.1038/nature00935

10.1016/S0958-1669(00)00075-6

10.1016/S0959-437X(03)00018-2

10.1038/nature01279

10.1038/nature01278

10.1038/418244a

C. I. Bargmann Genome Biol.2 REVIEWS1005 (2001).

10.1046/j.1365-313X.2001.01105.x

R. S. Kamath M. Martinez-Campos P. Zipperlen A. G. Fraser J. Ahringer Genome Biol.2 RESEARCH0002 (2001).

Materials and methods are available as supporting material on Science Online.

10.1093/genetics/159.4.1751

10.1105/tpc.11.12.2283

J. M. Alonso et al . unpublished observations.

10.1046/j.1365-313x.2001.01087.x

10.1016/S0962-8924(01)02229-2

10.1046/j.1364-3703.2000.00026.x

10.1016/S0092-8674(02)00864-4

10.1093/genetics/155.4.1875

10.1104/pp.123.3.895

10.1105/tpc.001768

10.1101/gad.12.23.3703

See http://signal.salk.edu

H. Chen J. R. Ecker unpublished observations.

P. Guzman, J. Ecker, Plant Cell2, 513 (1990)

We thank A. Hamilton and members of the Ecker laboratory for critical reading of the manuscript and stimulating discussions. J.M.A. was in part supported by a postdoctoral fellowship from the Spanish Ministerio de Educación y Ciencia. A.N.S. was a recipient of a predoctoral fellowship from the Department of Energy (ER20162-A0003). The W.L.C. laboratory was supported by a grant from the Canadian National Research Council Vice-President's fund. P. G. was on a sabbatical recess from Cinvestav-Irapuato Mexico and was supported in part by a fellowship from CONACYT Mexico. We thank K. Davis and colleagues at Paradigm Genetics for their assistance in bulking up low-seed T1 lines. This work was supported by an NSF Arabidopsis 2010 project grant (MCB O115103) and Department of Energy DE-FG03-00ER15113 awarded to J.R.E. Seeds for all the T-DNA lines are publicly available from The Arabidopsis Biological Resource Center at Ohio State University (http://arabidopsis.org/abrc/). Affymetrix microarray expression data has been deposited in the Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo/). GEO accessions: GSM8467 through GSM8478.

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 301 Số 5633

653-657

Thông tin tác giả