Genome Sequence of Aedes aegypti , a Major Arbovirus Vector

American Association for the Advancement of Science (AAAS) - Tập 316 Số 5832 - Trang 1718-1723 - 2007
Vishvanath Nene1,2,3,4,5, Jennifer R. Wortman1,2,3,4,5, Daniel Lawson1,2,3,5, Brian J. Haas1,2,3,4,5, Chinnappa D. Kodira1,2,3,4,5, Zhijian Tu1,2,3,4,5, Brendan Loftus1,2,3,4,5, Zhiyong Xi1,2,3,4,5, Karyn Mégy1,2,3,5, Manfred Grabherr1,2,3,4,5, Quinghu Ren1,2,3,4,5, Evgeny M. Zdobnov1,3,6,4,5, Neil F. Lobo1,2,3,4,5, Kathryn S. Campbell1,2,3,4,5, Susan E. Brown1,2,7,3,5, Maria F. Bonaldo1,2,3,4,5, Jinsong Zhu1,2,3,4,5, Steven P. Sinkins1,2,3,4,5, David G. Hogenkamp1,2,3,4,5, Paolo Amedeo1,2,3,4,5, Peter Arensburger1,2,3,4,5, Peter W. Atkinson1,2,3,4,5, Shelby Bidwell1,2,3,4,5, Jim Biedler1,2,3,4,5, Ewan Birney1,2,3,5, Robert V. Bruggner1,2,3,4,5, Javier Costas1,2,3,4,5, Monique R. Coy1,2,3,4,5, Jonathan Crabtree1,2,3,4,5, Matt Crawford1,2,3,4,5, Becky deBruyn1,2,3,4,5, David DeCaprio1,2,3,4,5, Karin Eiglmeier1,2,3,8,4,5, Eric Eisenstadt1,2,3,4,5, Hamza A. El-Dorry1,2,3,4,9,5, William M Gelbart1,2,3,4,5, Suely Lopes Gomes1,2,3,4,9,5, M. Hammond1,2,3,5, Linda I. Hannick1,2,3,4,5, James R. Hogan1,2,3,4,5, Megan E. Holmes1,2,3,4,5, David B. Jaffe1,2,3,4,5, J. Spencer Johnston1,2,3,4,5, Ryan Kennedy1,2,3,4,5, Hean Koo1,2,3,4,5, Saul Kravitz1,2,3,4,5, Evgenia V. Kriventseva1,2,3,4,5, David Kulp1,2,3,4,5, Kurt LaButti1,2,3,4,5, Eduardo Lee1,2,3,4,5, Li Song1,2,3,4,5, Diane D. Lovin1,2,3,4,5, Chunhong Mao1,2,3,4,5, Carlos Frederico Martins Menck1,2,3,10,5, Jason Miller1,2,3,4,5, Philip Montgomery1,2,3,4,5, Akio Mori1,2,3,4,5, Ana L. T. O. Nascimento1,2,3,11,4,5, Horacio Naveira1,2,3,4,5, Chad Nusbaum1,2,3,4,5, Sinéad B. O'Leary1,2,3,4,5, Joshua Orvis1,2,3,4,5, Mihaela Pertea1,2,3,4,5, Hadi Quesneville1,2,3,4,5, Kyanne R. Reidenbach1,2,3,4,5, Yu-Hui Rogers1,2,3,4,5, Charles W. Roth1,2,3,8,4,5, Jennifer R. Schneider1,2,3,4,5, Michael C. Schatz1,2,3,4,5, Martin Shumway1,2,3,4,5, Mario Stanke12,1,2,3,5, E. O. Stinson1,2,3,4,5, José M. C. Tubío1,2,13,3,4,5, Janice P. VanZee1,2,3,4,5, Sergio Verjovski‐Almeida1,2,3,4,9,5, Doreen Werner1,2,3,4,5, Owen White1,2,3,4,5, Stefan Wyder1,2,3,4,5, Qiandong Zeng1,2,3,4,5, Qi Zhao1,2,3,4,5, Yongmei Zhao1,2,3,4,5, Catherine A. Hill1,2,3,4,5, Alexander S. Raikhel1,2,3,4,5, Marcelo B. Soares1,2,3,4,5, D. L. Knudson1,2,7,3,5, Norman H. Lee1,2,3,4,5, James E. Galagan1,2,3,4,5, Steven L. Salzberg1,2,3,4,5, Ian T. Paulsen1,2,3,4,5, George Dimopoulos1,2,3,4,5, Frank H. Collins1,2,3,4,5, Claire M. Fraser1,2,3,4,5, David W. Severson1,2,3,4,5
1Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA
2Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02141, USA.
3European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD UK.
4The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
5Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
6Swiss Institute of Bioinformatics, 1 rue Michel-Servet, Geneva 1211, Switzerland.
7College of Agricultural Sciences, Colorado State University, Fort Collins, CO 80523, USA.
8Institut Pasteur, Paris 75724, France.
9Universidade de Sao Paulo, Instituto de Quimica, Sao Paulo SP 05508-900, Brazil.
10Universidade de Sao Paulo, Institute of Biomedical Sciences, Sao Paulo SP 05508-900, Brazil.
11Instituto Butantan, Sao Paulo SP 05503-900, Brazil.
12507A Engineering 2, University of California, 1156 High Street, Santa Cruz, CA 95064, USA.
13Complexo Hospitalario Universitario de Santiago, Santiago de Compostela 15706, Spain.

Tóm tắt

We present a draft sequence of the genome of Aedes aegypti , the primary vector for yellow fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae . Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of ∼4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster . Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of ∼2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.

Từ khóa


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The Aedes aegypti genome sequencing project at the microbial sequencing centers and VectorBase was funded by National Institute of Allergy and Infectious Diseases (NIAID) contracts HHSN266200309D266030071 HHSN266200400001C and HHSN266200400039C and was supported in part by NIAID grants UO1 AI50936 (D.W.S.) RO1 AI059492 (A.S.R. G.D.) 5 R01 AI61576-2 (G.D.) and R37 AI024716 (A.S.R.) and by Swiss National Science Foundation grant SNF 3100A0-112588/1 (E.M.Z.). We acknowledge the excellent work of the Broad Genome Sequencing Platform and the Venter Institute Joint Technology Center. We thank C. Town N. Hall and E. Kirkness for critical comments and the Aedes aegypti research community for their enthusiastic support and willing assistance in this project. On 1 October 2006 The Institute for Genomic Research merged with the J. Craig Venter Institute. The Ae. aegypti genome can also be accessed at VectorBase (http://aaegypti.vectorbase.org).

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American Association for the Advancement of Science (AAAS)

Tập 316 Số 5832

1718-1723

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