Mara Cecília Rosenzvit1, L.-H. Zhang2, Laura Kamenetzky1, Sergio G. Canova1, Eduardo Guarnera1, Donald P. McManus2
1Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, Administración Nacional de Laboratorios e Institutos de Salud #8216;Dr Carlos G. Malbrán#8217;, Vélez Sarsfield 563, 1281 Buenos Aires, Argentina
2QIMR Berghofer Medical Research Institute, Brisbane, Australia
Tóm tắt
Polymerase chain reaction–ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR–RFLP)
analysis and sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase 1 (ND1)
genes were used to characterize 33 Echinococcus granulosus isolates collected from different regions and hosts in Argentina,
and to determine which genotypes occurred in humans with cystic hydatid disease. The results of the study demonstrated
the presence of at least 4 distinct genotypes; the common sheep strain (G1) in sheep from Chubut Province and in humans
from Río Negro Province, the Tasmanian sheep strain (G2) in sheep and 1 human from Tucumán Province, the pig strain
(G7) in pigs from Santa Fe Province and the camel strain (G6) in humans from Río Negro and Buenos Aires Provinces.
The finding that pigs harboured the pig strain and the occurrence of the Tasmanian sheep strain has considerable
implications for the implementation of hydatid control programmes due to the shorter maturation time of both strains in
dogs compared with the common sheep strain. Furthermore, this is the first report of the presence of the G2 and G6
genotypes in humans which may also have important consequences for human health.