Genetic variability among group A and B respiratory syncytial viruses in Mozambique: identification of a new cluster of group B isolates

Journal of General Virology - Tập 82 Số 1 - Trang 103-111 - 2001
Anna Roca1,2, Mari‐Paz Loscertales1,2, Llorenç Quintó2, Pilar Pérez‐Breña3, Neide Vaz1, Pedro Alonso1,2, Juan‐Carlos Sáiz2
1Centro de Investigaçao en Saude da Manhiça, INS-Ministerio da Saude, Mozambique3
2Epidemiología y Bioestadística1 and Hepatología-IMD2, Fundació Clínic, IDIBAPS, Hospital Clínic, Universidad de Barcelona, Villaroel 170, 08036 Barcelona, Spain
3Servicio de Virología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain4

Tóm tắt

Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection in children and vulnerable adults, but little is known regarding RSV infection in Africa. In this report, a recent RSV outbreak in Mozambique was studied and results showed that 275 of 3192 (8·6%) nasopharyngeal aspirates tested were RSV-positive by ELISA. RSV presents two antigenic groups (A and B) with a high genetic and antigenic variability between and within them. Analysis by a new RFLP assay of RT–PCR amplified N protein gene products showed a higher prevalence of group B RSV than that of group A (85% versus 15%). However, genetic variability of the G protein gene was higher among group A RSV strains. The frequency and pattern of glycosylation sites were also quite different between both groups. In addition, two different phylogenetic clusters of Mozambican viruses were found within each group, but only sequences from cluster B-I were relatively distinct from previously described isolates. The implications of such differences in the antigenic and immunogenic characteristics of each group are discussed.

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Journal of General Virology

Tập 82 Số 1

103-111

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