Genetic transformation of Centaurium erythraea Rafn by Agrobacterium rhizogenes and the production of secoiridoids

Plant Cell Reports - Tập 25 - Trang 1308-1315 - 2006
Ewelina Piatczak1,2, Aleksandra Krolicka3, Halina Wysokinska1
1Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Lodz, Poland
2Department of Biology and Pharmaceutical Botany, Medical University of Wroclaw, Wroclaw, Poland
3Faculty of Biotechnology, Department of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland

Tóm tắt

Hairy roots of Centaurium erythraea were obtained by infection with Agrobacterium rhizogenes strain LBA 9402. They spontaneously regenerated adventitious shoots in Woody Plant liquid medium without growth regulators. The shoots were grown continuously in Murashige and Skoog (MS) liquid or agar solidified media supplemented with 0.1 mg l−1 indole-3-acetic acid and 1.0 mg l−1 6-benzylaminopurine. These shoots produced roots 4 weeks after transfer into agar-solidified MS medium without phytohormones. Regenerated plants grown and flowered under greenhouse conditions. The transgenic value of the regenerated plants was confirmed by the polymerase chain reaction amplification. Transformation by Agrobacterium rhizogenes alters plant morphology and production of secoiridoid glucosides. The level of secoiridoids was also modified by development stage of transformed plants. The total content of the compounds (expressed as the sum of gentiopicroside, sweroside and swertiamarin) in 10-week old pRi-transformed regenerants was 280 mg g−1 dry weight and was 8-times the content in the sample of commercially available C. erythraea herb.

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Plant Cell Reports

Tập 25

1308-1315

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