Genetic diversity revealed by phytochemical and molecular analyses among and within eight Trigonella sp.
Tóm tắt
Genetic diversity is a key factor for breeding programs and provides opportunities for developing superior varieties with preferred traits. Trigonella is a large genus of plants that is important in culinary terms and for the food industry. The present research aimed to study genetic diversity and population structure in a set of 90 accessions from eight different species of Trigonella sp. using 20 URP and SCoT primers. Also, five amino acids and Trigonelline content were determined in the plants. The average marker index was 3.43 and 3.73 for SCoT and URP primers, respectively. NJ clustering was based on combined data and classified all 90 accessions clearly, based on their species. AMOVA revealed that the main part (68%) of total variation occurring within species and T. monantha was the most diverse population among all species. According to the phytochemical, there were significant differences between all species in terms of trigonelline content and the amounts of amino acids. High levels of genetic diversity were identified among and within the eight species which can be considered for breeding purposes. Furthermore, URP and SCoT were found useful markers for the analysis of genetic diversity and population structures in Trigonella sp. Meanwhile, Trigonella foenum-graecum showed the highest Trigonelline content. As a matter of the health benefits provided by this alkaloid, T. foenum-graecum can be used not only for medicinal purposes but also as a useful vegetable and food ingredient.
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