Genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants

Journal of Basic Microbiology - Tập 48 Số 2 - Trang 118-124 - 2008
Encarna Velázquez1, Marcia M. Rojas Badía2, María J. Lorite3, Raúl Rivas1, José Luis Zurdo‐Piñeiro1, Mayra Heydrich2, Eulogio J. Bedmar3
1Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain
2Laboratorio de Ecología Microbiana, Facultad de Biología, Universidad de La Habana, La Habana, Cuba
3Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, C.S.I.C., Granada, Spain

Tóm tắt

Abstract

The genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in Cuba was analysed by Two Primers‐Ramdom Amplified Polymorphic DNA fingerprinting (TP‐RAPD) and 16S rRNA gene sequencing. The strains were distributed into 17 groups on the basis of their TP‐RAPD patterns, and a representative strain from each group was subjected to 16S rRNA gene sequencing. Analysis of these sequences showed that the isolates belong to a wide variety of phylogenetic groups being closely related to species of genera Bacillus and Staphylococcus from Firmicutes, Microbacterium, Micrococcus and Kokuria from Actinobacteria, Rhizobium and Gluconacetobacter from α ‐Proteobacteria, Comamonas and Xanthomonas from β ‐Proteobacteria, and Acinetobacter and Pantoea from γ ‐Proteobacteria. These results show the complexity of the bacterial populations present in inner tissues of sugarcane, and indicate the interest and relevance of the studies on microbial diversity to improve our knowledge on the plant endophytic bacterial communities. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Journal of Basic Microbiology

Tập 48 Số 2

118-124

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