Genetic control of lysine metabolism in maize endosperm mutants

Functional Plant Biology - Tập 31 Số 4 - Trang 339 - 2004
Ricardo Antunes Azevedo1, Catherine Damerval2, Peter J. Lea3, Jacques Landry4, Cláudia de M. Bellato5, Lyndel W. Meinhardt6, Martine Le Guilloux2, Sonia Delhaye4, Alejandro Toro7, Salete Aparecida Gaziola7, Vanderlei Aparecido Varisi7, Priscila Lupino Gratão7
1Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba CEP 13418-900, SP, Brazil. Corresponding author; email: [email protected].
2Station de Génétique Végétale INRA / UPS / INA-PG / CNRS UMR 8120, La Ferme du Moulon, 91190 Gif-sur-Yvette, France.
3Department of Biological Sciences, University of Lancaster, Lancaster LA1 4YQ, United Kingdom
4INRA, Laboratoire de Chimie Biologique, INA-PG, F78850 Thiverval-Grignon, France.
5Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba CEP 13418-900, SP, Brazil.
6Departamento de Genética e Evolução, Universidade Estadual de Campinas, Campinas CEP 13081-970, Brazil.
7Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba CEP 13418-900, SP, Brazil

Tóm tắt

The capacity of three maize endosperm opaque mutants (o10, o11 and o13) to accumulate soluble lysine in the seed in relation to their wildtype counterpart, W22+, was investigated. The W22o13 and W22o11 mutants exhibited 278% and 186% increases in soluble lysine, respectively, while for W22o10, a 36% decrease was observed, compared with the wildtype. A quantitative and qualitative study of the N constituents of the endosperm has been conducted and data obtained for the total protein, non-protein N, soluble amino acids, albumins / globulins, zeins and glutelins present in the seed of the mutants. Following 2D–PAGE, a total of 38 different forms of zein polypeptides were detected and considerable differences were noted between the three mutant lines. The metabolism of lysine was also studied by analysis of the enzymes aspartate kinase, homoserine dehydrogenase, lysine 2-oxoglutarate reductase and saccharopine dehydrogenase, which exhibited major changes in activity, depending on the genotype, suggesting that the mutant genes may have distinct regulatory activities.

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Functional Plant Biology

Tập 31 Số 4

339

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