Genetic Transformation of Drosophila with Transposable Element Vectors

American Association for the Advancement of Science (AAAS) - Tập 218 Số 4570 - Trang 348-353 - 1982
Gerald M. Rubin1, Allan C. Spradling1
1Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210

Tóm tắt

Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos. Transformants contained one or two copies of chromosomally integrated, intact ry1 that were stably inherited in subsequent generations. These transformed flies had wild-type eye color indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene. To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes.

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American Association for the Advancement of Science (AAAS)

Tập 218 Số 4570

348-353

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