Genetic Diversity Analysis Reveals that Geographical Environment Plays a More Important Role than Rice Cultivar in Villosiclava virens Population Selection

Applied and Environmental Microbiology - Tập 80 Số 9 - Trang 2811-2820 - 2014
Fei Wang1, Shu Zhang2, Mei-Gang Liu3, Xian-Song Lin4, Hui-Jiang Liu4, You‐Liang Peng5, Lin Yang6, Junbin Huang6, Chaoxi Luo6
1Department of Plant Protection, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, China.
2bInstitute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Science, Wuhan, China
3cPlant Protection Station of Xiantao City, Xiantao, China
4dPlant Protection Station of Yangxin County, Yangxin, China
5eDepartment of Plant Pathology, College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
6aDepartment of Plant Protection, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, China

Tóm tắt

ABSTRACT Rice false smut caused by Villosiclava virens is an economically important disease of grains worldwide. The genetic diversity of 153 isolates from six fields located in Wuhan (WH), Yichang Wangjia (YCW), Yichang Yaohe (YCY), Huanggang (HG), Yangxin (YX), and Jingzhou (JZ) in Hubei province of China were phylogenetically analyzed to evaluate the influence of environments and rice cultivars on the V. virens populations. Isolates (43) from Wuhan were from two rice cultivars, Wanxian 98 and Huajing 952, while most of the other isolates from fields YCW, YCY, HG, YX, and JZ originated from different rice cultivars with different genetic backgrounds. Genetic diversity of isolates was analyzed using random amplified polymorphic DNA (RAPD) and single-nucleotide polymorphisms (SNP). The isolates from the same cultivars in Wuhan tended to group together, indicating that the cultivars had an important impact on the fungal population. The 110 isolates from individual fields tended to cluster according to geographical origin. The values of Nei's gene diversity ( H ) and Shannon's information index ( I ) showed that the genetic diversity among isolates was higher between than within geographical populations. Furthermore, mean genetic distance between groups (0.006) was higher than mean genetic distance within groups (0.0048) according to MEGA 5.2. The pairwise population fixation index ( F ST ) values also showed significant genetic differentiation between most populations. Higher genetic similarity of isolates from individual fields but different rice cultivars suggested that the geographical factor played a more important role in the selection of V. virens isolates than rice cultivars. This information could be used to improve the management strategy for rice false smut by adjusting the cultivation measures, such as controlling fertilizer, water, and planting density, in the rice field to change the microenvironment.

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Applied and Environmental Microbiology

Tập 80 Số 9

2811-2820

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