Genetic Analysis of a High-Level Vancomycin-Resistant Isolate of Staphylococcus aureus

American Association for the Advancement of Science (AAAS) - Tập 302 Số 5650 - Trang 1569-1571 - 2003
Linda M. Weigel1,2,3,4, Don B. Clewell1,2,3,4, Steven R. Gill1,2,3,4, Nancye C. Clark1,2,3,4, Linda K. McDougal1,2,3,4, Susan E. Flannagan1,2,3,4, James F. Kolonay1,2,3,4, Jyoti Shetty1,2,3,4, George Killgore1,2,3,4, Fred C. Tenover1,2,3,4
1Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA
2Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
3J. Craig Venter Science Foundation Joint Technology Center, Rockville, MD 20850, USA.
4The Institute for Genomic Research, Rockville, MD 20850, USA

Tóm tắt

Vancomycin is usually reserved for treatment of serious infections, including those caused by multidrug-resistant Staphylococcus aureus . A clinical isolate of S. aureus with high-level resistance to vancomycin (minimal inhibitory concentration = 1024 μg/ml) was isolated in June 2002. This isolate harbored a 57.9-kilobase multiresistance conjugative plasmid within which Tn 1546 ( vanA ) was integrated. Additional elements on the plasmid encoded resistance to trimethoprim ( dfrA ), β-lactams ( blaZ ), aminoglycosides ( aacA-aphD ), and disinfectants ( qacC ). Genetic analyses suggest that the long-anticipated transfer of vancomycin resistance to a methicillin-resistant S. aureus occurred in vivo by interspecies transfer of Tn 1546 from a co-isolate of Enterococcus faecalis .

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We thank J. B. Patel and J. K. Rasheed for helpful discussions and critical reading of the manuscript. DNA sequencing and annotation of pLW1043 performed by The Institute for Genomic Research (TIGR) was funded by the National Institute of Allergy and Infectious Diseases NIH (Network on Antimicrobial Resistance in Staphylococcus aureus contract N01-AI-95359).

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American Association for the Advancement of Science (AAAS)

Tập 302 Số 5650

1569-1571

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