Generation of hydrogen peroxide in resting and activated platelets

Cell Biochemistry and Function - Tập 10 Số 2 - Trang 79-85 - 1992
Marc Maresca1, C Colao1, Giuliana Leoncini1
1Istituto Policattedra di Chimica Biologica, Universitá di Genova, Italy

Tóm tắt

AbstractThe production of hydrogen peroxide was measured by following the oxidation of dichlorofluorescein (DCFH) entrapped into platelets. Resting platelets produced nanomolar quantities of DCF, which was proportional to the concentration of platelets and was steady during 1 h of incubation. A significant increase of basal DCF fluorescence was induced by stimuli namely thrombin, arachidonic acid, the Ca2+ ionophore A23187 and PMA. The effect of agonists has been also measured in the presence of 3‐amino‐1,2,4‐triazole (AT) or N‐ethylmaleimide (NEM), inhibitors of catalase and glutathione peroxidase, respectively. A further significant enhancement of DCF produced in stimulated platelets was detected only in the presence of NEM. A correlation was found between the increase in DCF and externally added hydrogen peroxide or the oxidizing species formed by xanthine oxidase plus acetaldehyde. The yield was not affected by superoxide dismutase and was higher in the presence of AT or NEM. A cooperative effect in the presence of both inhibitors was shown. Glutathione peroxidase plus glutathione diminished the level of DCF to basal levels.

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Cell Biochemistry and Function

Tập 10 Số 2

79-85

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