Generation of Neurons and Astrocytes from Isolated Cells of the Adult Mammalian Central Nervous System

American Association for the Advancement of Science (AAAS) - Tập 255 Số 5052 - Trang 1707-1710 - 1992
Brent A. Reynolds1, Samuel Weiss1
1Neuroscience Research Group, Department of Pathology, and Department of Pharmacology and Therapeutics, University of Calgary Faculty of Medicine, Calgary, Alberta, Canada T2N 4N1.

Tóm tắt

Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth; in the mouse striatum no new neurons are produced after the first few days after birth. In this study, cells isolated from the striatum of the adult mouse brain were induced to proliferate in vitro by epidermal growth factor. The proliferating cells initially expressed nestin, an intermediate filament found in neuroepithelial stem cells, and subsequently developed the morphology and antigenic properties of neurons and astrocytes. Newly generated cells with neuronal morphology were immunoreactive for γ-aminobutyric acid and substance P, two neurotransmitters of the adult striatum in vivo. Thus, cells of the adult mouse striatum have the capacity to divide and differentiate into neurons and astrocytes.

Từ khóa


Tài liệu tham khảo

ANCHAN, R.M., EGF AND TGF-ALPHA STIMULATE RETINAL NEUROEPITHELIAL CELL-PROLIFERATION INVITRO, NEURON 6: 923 (1991).

BAYER, S.A., NEURON PRODUCTION IN THE HIPPOCAMPUS AND OLFACTORY-BULB OF THE ADULT-RAT BRAIN - ADDITION OR REPLACEMENT, ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 457: 163 (1985).

BIRECREE, E, EPIDERMAL GROWTH-FACTOR AND ITS RECEPTOR IN THE DEVELOPING HUMAN NERVOUS-SYSTEM, DEVELOPMENTAL BRAIN RESEARCH 60: 145 (1991).

Carpenter, G., Peptide Growth Factors and Their Receptors 1: 69 (1990).

CATTANEO, E, PROLIFERATION AND DIFFERENTIATION OF NEURONAL STEM-CELLS REGULATED BY NERVE GROWTH-FACTOR, NATURE 347: 762 (1990).

CATTANEO, E, IDENTIFYING AND MANIPULATING NEURONAL STEM-CELLS, TRENDS IN NEUROSCIENCES 14: 338 (1991).

10.1126/science.6144184

FENTRESS, J.C., OBSERVATIONS ON THE DEVELOPMENT OF THE STRIATUM IN MICE AND RATS, ANATOMY AND EMBRYOLOGY 163: 275 (1981).

FREDERIKSEN, K, JOURNAL OF NEUROSCIENCE 8: 1144 (1988).

GENSBURGER, C, BRAIN BASIC FIBROBLAST GROWTH-FACTOR STIMULATES THE PROLIFERATION OF RAT NEURONAL PRECURSOR CELLS-INVITRO, FEBS LETTERS 217: 1 (1987).

GOMEZPINILLA, F, EPIDERMAL GROWTH-FACTOR RECEPTOR IMMUNOREACTIVITY IN RAT-BRAIN - DEVELOPMENT AND CELLULAR-LOCALIZATION, BRAIN RESEARCH 438: 385 (1988).

GRAYBIEL, A.M., NEUROTRANSMITTERS AND NEUROMODULATORS IN THE BASAL GANGLIA, TRENDS IN NEUROSCIENCES 13: 244 (1990).

Jacobsen M. Developmental Neurobiology 3: 41 (1991).

KAY, A.R., ISOLATION OF NEURONS SUITABLE FOR PATCH-CLAMPING FROM ADULT MAMMALIAN CENTRAL NERVOUS SYSTEMS, JOURNAL OF NEUROSCIENCE METHODS 16: 227 (1986).

LENDAHL, U, CNS STEM-CELLS EXPRESS A NEW CLASS OF INTERMEDIATE FILAMENT PROTEIN, CELL 60: 585 (1990).

MORRISON, R.S., TROPHIC STIMULATION OF CULTURED NEURONS FROM NEONATAL RAT-BRAIN BY EPIDERMAL GROWTH-FACTOR, SCIENCE 238: 72 (1987).

MORSHEAD, C.M., POSTMITOTIC DEATH IS THE FATE OF CONSTITUTIVELY PROLIFERATING CELLS IN THE SUBEPENDYMAL LAYER OF THE ADULT-MOUSE BRAIN, JOURNAL OF NEUROSCIENCE 12: 249 (1992).

MURPHY, M, FIBROBLAST GROWTH-FACTOR STIMULATES THE PROLIFERATION AND DIFFERENTIATION OF NEURAL PRECURSOR CELLS-INVITRO, JOURNAL OF NEUROSCIENCE RESEARCH 25: 463 (1990).

REYNOLDS B.A. unpublished data.

SIMPSON, D.L., EPIDERMAL GROWTH-FACTOR BINDING AND MITOGENIC ACTIVITY ON PURIFIED POPULATIONS OF CELLS FROM THE CENTRAL NERVOUS-SYSTEM, JOURNAL OF NEUROSCIENCE RESEARCH 8: 453 (1982).

WEISS, S, SYNAPTOGENESIS OF CULTURED STRIATAL NEURONS IN SERUM-FREE MEDIUM - A MORPHOLOGICAL AND BIOCHEMICAL-STUDY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 2238 (1986).

WERNER, M.H., LOCALIZATION OF IMMUNOREACTIVE EPIDERMAL GROWTH-FACTOR RECEPTORS IN HUMAN NERVOUS-SYSTEM, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 36: 81 (1988).

WILLIAMS, J.S., N-METHYL-D-ASPARTATE EVOKES THE RELEASE OF SOMATOSTATIN FROM STRIATAL INTERNEURONS IN PRIMARY CULTURE, NEUROSCIENCE 43: 437 (1991).

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 255 Số 5052

1707-1710

Thông tin tác giả