Gene silencing mechanisms mediated by Aubergine–piRNA complexes in <i>Drosophila</i> male gonad

RNA - Tập 13 Số 11 - Trang 1911-1922 - 2007
Kazumichi M. Nishida1, Kuniaki Saito1, Tomoko Mori1, Yoshinori Kawamura1, Tomoko Nagami-Okada1, Sachi Inagaki1, Haruhiko Siomi1, Mikiko C. Siomi1
1Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan

Tóm tắt

Genetic studies have shown that Aubergine (Aub), one of the Piwi subfamily of Argonautes in Drosophila, is essential for germ cell formation and maintaining fertility. aub mutations lead to the accumulation of retrotransposons in ovaries and testes, and Stellate transcripts in testes. Aub in ovaries associates with a variety of Piwi-interacting RNAs (piRNAs) derived from repetitive intergenic elements including retrotransposons. Here we found that Aub in testes also associates with various kinds of piRNAs. Although in ovaries Aub-associated piRNA populations are quite diverse, piRNAs with Aub in testes show a strong bias. The most abundant piRNAs were those corresponding to antisense transcripts of Suppressor of Stellate [Su(Ste)] genes known to be involved in Stellate gene silencing. The second most abundant class was made up of those from chromosome X and showed strong complementarity to vasa transcripts. Immunopurified Aub–piRNA complexes from testes displayed activity in cleaving target RNA containing sequences complementary to Stellate and vasa transcripts. These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes.

Từ khóa


Tài liệu tham khảo

10.1038/nature02871

10.1016/S0960-9822(01)00299-8

10.1016/S1534-5807(03)00228-4

10.1128/MCB.24.15.6742-6750.2004

10.1093/nar/20.14.3731

10.1073/pnas.92.13.6067

10.1016/j.cell.2006.12.025

10.1101/gad.1026102

Cox,, 1998, A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal, Genes & Dev., 12, 3715, 10.1101/gad.12.23.3715

Cox,, 2000, piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells, Development, 127, 503, 10.1242/dev.127.3.503

10.1242/dev.02070

Eddy,, 1975, Germ plasm and the differentiation of the germ cell line, Int. Rev. Cytol., 43, 229, 10.1016/S0074-7696(08)60070-4

10.1101/gad.1456106

10.1242/dev.00310

10.1038/35888

10.1016/j.cell.2007.05.056

Girard,, 2006, A germline-specific class of small RNAs binds mammalian Piwi proteins, Nature, 442, 199, 10.1038/nature04917

10.1126/science.1140494

Harris,, 2001, Aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C, Development, 128, 2823, 10.1242/dev.128.14.2823

10.1101/gad.1022002

10.1093/nar/gki323

10.1101/gad.990802

10.1261/rna.283207

10.1016/j.devcel.2006.12.001

10.1016/j.devcel.2006.09.009

10.1038/335611a0

10.1038/sj.embor.7400637

Lin,, 1997, A novel group of pumilio mutations affects the asymmetric division of germline stem cells in the Drosophila ovary, Development, 124, 2463, 10.1242/dev.124.12.2463

10.1126/science.1088710

10.1083/jcb.45.2.306

10.1016/j.cell.2005.08.044

10.1101/gad.1370605

10.1016/j.ceb.2004.09.003

10.1101/gad.1210204

10.1016/j.tibs.2006.09.010

10.1016/j.cell.2005.10.020

10.1101/gad.1454806

10.1101/gad.1563607

10.1016/S0925-4773(01)00654-2

10.1534/genetics.166.3.1313

10.1101/gad.370206

Schmidt,, 1999, Genetic and molecular characterization of sting, a gene involved in crystal formation and meiotic drive in the male germ line of Drosophila melanogaster, Genetics, 151, 749, 10.1093/genetics/151.2.749

10.1016/j.cub.2004.03.008

10.1242/jcs.01059

Styhler,, 1998, vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development, Development, 125, 1569, 10.1242/dev.125.9.1569

10.1016/j.cub.2005.01.005

10.1101/gad.1284105

10.1126/science.1102755

10.1016/j.cell.2007.05.057

Vagin,, 2004, The RNA interference proteins and Vasa locus are involved in the silencing of retrotransposons in the female germline of Drosophila melanogaster, RNA Biol., 1, 54, 10.4161/rna.1.1.943

10.1126/science.1129333

10.1073/pnas.072190799

Wilson,, 1996, aubergine enhances oskar translation in the Drosophila ovary, Development, 122, 1631, 10.1242/dev.122.5.1631

Zamore,, 2007, RNA silencing: Genomic defence with a slice of pi, Nature, 19, 864, 10.1038/446864a

Zaratiegui,, 2007, Noncoding RNAs and gene silencing, Cell, 23, 763, 10.1016/j.cell.2007.02.016

Thông tin
Thông tin xuất bản

RNA

Tập 13 Số 11

1911-1922

Thông tin tác giả