Furfural gây ra sự tích lũy các loài oxy phản ứng và tổn thương tế bào ở Saccharomyces cerevisiae

Biotechnology for Biofuels - Tập 3 Số 1 - 2010
Sandra A Allen1, William G. Clark1, J. Michael McCaffery2, Zhen Cai1, Alison A. Lanctot1, Patricia J. Slininger3, Z. Lewis Liu3, Steven Ward Gorsich1
1Biology Department, Central Michigan University, Mt Pleasant, USA
2Integrated Imaging Center, Department of Biology, Johns Hopkins University, Baltimore, USA
3Agricultural Research Service, United States Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, USA

Tóm tắt

Tóm tắt Đặt vấn đề Sinh khối sinh học cung cấp một lựa chọn khả thi thay thế cho nhiên liệu dựa trên dầu mỏ. Tuy nhiên, các phương pháp hiện tại chưa đủ hiệu quả và công nghệ cần thiết để sử dụng sinh khối lignocellulosic như một nền tảng lên men gặp nhiều thách thức. Một trong những thách thức là cần có một vi sinh vật lên men mạnh mẽ có khả năng chịu đựng các chất ức chế có mặt trong quá trình lên men lignocellulosic. Những chất ức chế này bao gồm furan aldehyde, furfural, được giải phóng như một sản phẩm phụ của sự khử nước pentose trong quá trình xử lý axit yếu của lignocellulose. Để tồn tại trong môi trường có mặt furan, các tế bào nấm men không chỉ cần giảm furfural thành furan methanol ít độc hại hơn mà còn phải tự bảo vệ và sửa chữa bất kỳ tổn thương nào do furfural gây ra. Vì khả năng chịu đựng furfural ở nấm men yêu cầu một con đường phốt phát pentose (PPP) hoạt động, và PPP liên quan đến khả năng chịu đựng các loài oxy phản ứng (ROS), chúng tôi quyết định điều tra liệu furfural có kích thích ROS và tổn thương tế bào liên quan hay không ở nấm men.

Từ khóa


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Biotechnology for Biofuels

Tập 3 Số 1

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