Functional screening for salinity tolerant genes from Acanthus ebracteatus Vahl using Escherichia coli as a host

Trees - Tập 21 - Trang 515-520 - 2007
P. D. Nguyen1,2, C.-L. Ho3, J. A. Harikrishna4, M. C. V.-L. Wong5, R. Abdul Rahim6
1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM-Serdang, Malaysia
2College of Agriculture and Applied Biology, CanTho Univerisity, CanTho City, Vietnam
3Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), Serdang, Malaysia
4Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
5Biotechnology Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia
6Department Bioprocess, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Malaysia

Tóm tắt

Salinity reduces plant growth and crop production globally. The discovery of genes in salinity tolerant plants will provide the basis for effective genetic engineering strategies, leading to greater stress tolerance in economically important crops. In this study, we have identified and isolated 107 salinity tolerant candidate genes from a mangrove plant, Acanthus ebracteatus Vahl by using bacterial functional assay. Sequence analysis of these putative salinity tolerant cDNA candidates revealed that 65% of them have not been reported to be stress related and may have great potential for the elucidation of unique salinity tolerant mechanisms in mangrove. Among the genes identified were also genes that had previously been linked to stress response including salinity tolerance, verifying the reliability of this method in isolating salinity tolerant genes by using E. coli as a host.

Tài liệu tham khảo

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