Functional characterization of cytochrome P450 monooxygenases in the cereal head blight fungus Fusarium graminearum

Wiley - Tập 19 Số 5 - Trang 2053-2067 - 2017
Ji Young Shin1, Duc‐Cuong Bui2, Yoonji Lee1, Hyejin Nam1, Soyun Jung1, Miao Fang1, Jin‐Cheol Kim3, Theresa Lee4, Hun Kim5, Gyung Ja Choi5, Hokyoung Son2, Yin‐Won Lee1
1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea
2Center for Food and Bioconvergence, Seoul National University, Seoul, 08826 Republic of Korea
3Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186 Republic of Korea
4Microbial Safety Team, National Academy of Agricultural Science, Rural Development Administration, Wanju, 55365 Republic of Korea
5Eco-friendly New Materials Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, 34114 Republic of Korea

Tóm tắt

Summary

Fusarium graminearum is a prominent plant pathogenic fungus causing Fusarium head blight in major cereal crops worldwide. To understand the molecular mechanisms underlying fungal development and virulence, large collections of F. graminearum mutants have been constructed. Cytochrome P450 monooxygenases (P450s) are widely distributed in organisms and are involved in a diverse array of molecular/metabolic processes; however, no systematic functional analysis of P450s has been attempted in filamentous fungi. In this study, we constructed a genome‐wide deletion mutant set covering 102 P450s and analyzed these mutants for changes in 38 phenotypic categories, including fungal development, stress responses and responses to several xenobiotics, to build a comprehensive phenotypic dataset. Most P450 mutants showing defective phenotypes were impaired in a single phenotypic trait, demonstrating that our mutant library is a good genetic resource for further fungal genetic studies. In particular, we identified novel P450s specifically involved in virulence (5) and both asexual (1) and sexual development (2). Most P450s seem to play redundant roles in the degradation of xenobiotics in F. graminearum. This study is the first phenome‐based functional analysis of P450s, and it provides a valuable genetic resource for further basic and applied biological research in filamentous fungi and other plant pathogens.

Từ khóa


Tài liệu tham khảo

10.1007/PL00000897

10.1021/acs.jnatprod.5b00676

10.1128/aem.63.7.2495-2501.1997

10.1016/S0031-9422(00)89567-9

10.1094/PHYTO.1999.89.2.182

10.1006/fgbi.1997.1021

10.1006/fgbi.2001.1256

10.1002/bies.950060405

10.4148/1941-4765.1150

10.1007/BF00402301

10.1094/MPMI-05-11-0119

10.1073/pnas.0601456103

10.1016/j.bbapap.2010.06.020

10.1099/00221287-143-6-1993

10.1371/journal.pone.0058008

10.1016/j.ijfoodmicro.2007.07.024

10.1007/s00284-005-4480-2

10.1111/nph.12193

10.1007/s12275-016-5620-z

10.1038/nature10947

10.1007/s002030050627

10.1016/j.fgb.2006.11.001

10.1016/j.funbio.2015.10.010

10.1016/j.taap.2005.01.030

10.1038/ncomms7757

10.1002/bies.10083

10.1046/j.1523-1747.2000.00876.x

10.1038/nbt.1628

10.1271/bbb.70183

10.1038/sj.emboj.7600121

10.1038/ncomms12766

10.1128/EC.00335-08

10.1002/9780470278376

10.1371/journal.pone.0025586

10.1016/j.fgb.2010.10.004

10.1006/meth.2001.1262

10.1094/MPMI-03-10-0075

10.1016/S0742-8413(98)10026-9

10.1016/S0901-5027(96)80060-9

10.1093/nar/gkv468

10.1093/oxfordjournals.jbchem.a021260

10.1094/MPMI.2001.14.4.580

10.1097/00008571-199602000-00002

10.1534/genetics.166.1.113

10.1186/1471-2164-9-402

Paxton J., 1980, A new working definition of the term “phytoalexin, Plant Dis, 64, 734

10.1016/0958-1669(95)80054-9

10.2217/fmb.12.102

Sambrook J., 2001, Molecular Cloning: A Laboratory Manual

10.1016/S1368-7646(98)80006-X

10.1007/BF00437050

10.1016/j.fgb.2012.05.009

10.1016/j.fgb.2011.01.002

10.1371/journal.ppat.1002310

10.1371/journal.pone.0072915

10.1371/journal.pone.0155671

10.1021/cr9500500

10.1007/BF01576368

10.1155/2012/713687

10.1371/journal.ppat.1002460

10.1186/gb-2000-1-6-reviews3003

10.1104/pp.126.2.485

10.1126/science.285.5429.901

10.1016/j.fgb.2004.08.001

10.1111/nph.13374

10.1371/journal.pone.0045432

Thông tin
Thông tin xuất bản

Wiley

Tập 19 Số 5

2053-2067

Thông tin tác giả