Free radical scavenging systems in developing rat brain

International Journal of Developmental Neuroscience - Tập 9 Số 2 - Trang 181-185 - 1991
B. R. Shivakumar1, Hindupur K. Anandatheerthavarada1, Vijayalakshmi Ravindranath1
1Department of Neurochemistry, National Institute of Mental Health & Neuro Sciences, Bangalore, India

Tóm tắt

AbstractBecause the developing brain is subject to high oxygen tension and lacks a functional blood‐brain barrier, anti‐oxidant protection is important to development in the brain. The levels of superoxide dismutase, copper‐zinc superoxide dismutase. manganese superoxide dismutase. catalase, glutathione and related enzymes, namely, glutathione reductase and glutathione peroxidase were determined in rat brain at various stages of development. The levels of thiobarbituric acid reactive products, indicative of lipid peroxidation, were very low at birth and increased to adult levels by the 16th day after birth. Brain glutathione levels displayed significant variations during the first 2 weeks after birth but not thereafter. Catalase activity in developing brain slowly increased over 45 days. Total superoxide dismutase activity in 1‐day‐old rat brain, 80% of the adult rat brain level, subsequently decreased on day 6. Total superoxide dismutase activity, however, increased again in 10‐day‐old rats and remained constant thereafter. While the developmental pattern of manganese superoxide dismutase was similar to that of the total superoxide dismutase, the copper‐zinc superoxide dismutase levels were low at birth and reached adult levels on the 10th day after birth. There was no variation in glutathione reductase and peroxidase levels except for a decrease on day 16 of glutathione reductase and slow increase in adult levels by day 28. The present findings suggest that the overall levels of antioxidant enzymes in the developing brain are comparable to a large extent to those present in the adult brain. In contrast to the developing brain, hepatic levels of glutathione, total superoxide dismutase, manganese superoxide dismutase are significantly lower at birth and increase during development.

Từ khóa


Tài liệu tham khảo

10.1007/BF01268883

10.1016/0003-2697(76)90527-3

10.1016/0047-6374(87)90051-0

Freeman B.A., 1982, Biology of disease, free radicals and tissue injury, Lab. Invest., 47, 412

Fridovich I., 1974, Superoxide dismutases, 35

10.1126/science.210504

10.1016/0309-1651(78)90032-2

Horn H.D., 1965, Glutathione reductase, Methods in Enzymatic Analysis, 1, 875, 10.1016/B978-0-12-395630-9.50156-0

10.1016/0166-2236(85)90010-4

Kellog E.W., 1977, Liposome oxidation and hydrogen peroxide, J. Biol. Chem., 252, 6721, 10.1016/S0021-9258(17)39909-X

Larner A.J., 1989, Free radicals in acute myocardial infarction, Q.J. Med., 70, 205

10.1016/0024-3205(89)90365-2

Maker H.S., 1983, Glutathione, Handbook of Neurochemistry, 3, 607

Marttila R.J., 1989, Parkinsonism and Aging, 89

10.1007/BF01245142

10.1016/0003-2697(79)90738-3

10.1016/S0076-6879(84)05064-3

10.1016/0304-3940(89)90528-4

Reed D.J., 1984, Glutathione depletion and susceptibility, Pharmacol. Rev., 36, 255

10.1139/y82-200

10.1111/j.1471-4159.1980.tb11222.x

10.1042/bj2220001

10.1016/0003-2697(69)90064-5

Wendel A., 1989, Glutathione peroxidase, 325

Thông tin
Thông tin xuất bản

International Journal of Developmental Neuroscience

Tập 9 Số 2

181-185

Thông tin tác giả