Formulation of enamel matrix derivative for surface coating

Journal of Clinical Periodontology - Tập 24 Số 9 - Trang 678-684 - 1997
Stina Gestrelius1, Christer Andersson2, Ann‐Christin Johansson2, Eva Persson2, A Brodin3, L. Rydhag3, Lars Hammarström4
1Biora AB, Malmö, Sweden,
2BIORA AB, Malmö, Karolinska Institute, Stockholm, Sweden
3Astra Pain Control AB, Södertälje, Karolinska Institute, Stockholm, Sweden
4Center for Oral Biology, Karolinska Institute, Stockholm, Sweden

Tóm tắt

Abstract Enamel Matrix Derivative (EMD) contains a protein complex belonging to the amelogenin family. Enamel matrix as well as EMD have been found to promote periodontal regeneration when applied onto denuded root surfaces in dehiscence models. In the present studies it is shown that propylene glycol alginate (PGA) is a suitable vehicle for EMD for its local application. EMD can be dissolved in PGA at an acidic pH, resulting in a highly viscous solution. At neutral pH and body temperature the viscosity decreases and EMD precipitates. Multilayers of EMD on mineral or protein surfaces have been analysed using ellipsometry, total internal reflection fluorescence (TIRE) and biospecific interaction analysis (BIA). The studies show that EMD adsorbs both to hydroxyapatite and collagen and to denuded dental roots. It forms insoluble spherical complexes, and detectable amounts remain at the site of application on the root surface for two weeks, as shown with radiolabelled protein in rats and pigs. Scanning electron micrograph (SEM) studies on monkey teeth further indicate that EMD in PGA may promote repopulation of fibroblast‐like cells during the first weeks after application.

Từ khóa


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Thông tin xuất bản

Journal of Clinical Periodontology

Tập 24 Số 9

678-684

Thông tin tác giả