Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

IUBMB Life - Tập 61 Số 11 - Trang 1029-1042 - 2009
Jörg Wiedenmann1,2, Franz Oswald3, G. Ulrich Nienhaus4,5,6
1National Oceanography Centre, University of Southampton, Southampton, UK
2Tel: +44 (0)23 8059 6497.
3Department of Internal Medicine I, University of Ulm, Ulm, Germany
4Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA
5Institute of Applied Physics and Center for Functional Nanostructures, University of Karlsruhe, Karlsruhe, Germany
6Institute of Biophysics, University of Ulm, Ulm, Germany

Tóm tắt

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP‐like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes. © 2009 IUBMB IUBMB Life, 61(11): 1029–1042, 2009

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IUBMB Life

Tập 61 Số 11

1029-1042

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