Fluorescent Pseudomonas species categorized by using polymerase chain reaction (PCR)/restriction fragment analysis of 16S rDNA

Molecular Ecology - Tập 3 Số 5 - Trang 479-487 - 1994
Gisèle Laguerre1, Lionel Rigottier‐Gois2, Philippe Lemanceau2
1Laboratoire de Microbiologie des Sols, INRA, Dijon, France
2*Laboratoire de Recherches sur la Flore Pathogene du Sol, INRA, 17, rue Sully, B.V. 1540, 21034 Dijon Cedex, France

Tóm tắt

Abstract

A rapid procedure for the identification of fluorescent pseudomonads, based on the polymerase chain reaction (PCR) and restriction fragment analysis of 16S rDNA genes is described. Thirty‐one strains belonging to 10 different Pseudomonas species of the Pseudomonas fluorescens rRNA branch were characterized. Amplified rDNA was digested with 13 different restriction endonucleases. The combined data from restriction analysis enabled the definition of 17 different 16S rDNA genotypes. All type strains belonging to different species were differentiated. The good correlation between grouping obtained using restriction analysis with other molecular classification criteria demonstrates the value of the described method to characterize rapidly fluorescent Pseudomonas strains at the species level.

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Tài liệu tham khảo

Barrett EJ, 1986, Pseudomonas fluorescens biovar V: its resolution into distinct groups and the relationships of these groups to other P. fluorescens biovars, to P. putida, and to psychrotrophic pseudomonads associated with food spoliage, Journal of General Microbiology, 132, 2709

Bruijn FJ, 1992, Use of repetitive (Repetitive Extragenic Palindromic and Enterobacterial Repetitive Intergenic Consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria, Applied and Environmental Microbiology, 58, 2180, 10.1128/aem.58.7.2180-2187.1992

Champion AB, 1980, Evolution in Pseudomonas fluorescens, Journal of General Microbiology, 120, 485

10.1099/00207713-33-3-487

10.1099/00207713-35-2-169

10.1099/00207713-39-1-35

10.1099/00207713-42-4-606

10.1111/j.1365-2958.1991.tb01985.x

10.1099/00221287-137-11-2673

Haynes WC, 1957, Bergey's Manual of Determinative Bacteriology, 89

10.1073/pnas.86.23.9355

Jayarao BM, 1992, Restriction fragment length polymorphism analysis of 16S ribosomal DNA of Streptococcus and Enterococcus species of bovine origin, Journal of Clinical Microbiology, 30, 2235, 10.1128/jcm.30.9.2235-2240.1992

Jensen MA, 1993, Rapid identification of bacteria on the basis of polymerase chain reaction‐amplified ribosomal DNA spacer polymorphisms, Applied and Environmental Microbiology, 59, 945, 10.1128/aem.59.4.945-952.1993

10.1099/00207713-39-3-230

10.1016/B978-1-4832-3211-9.50009-7

10.1094/Phyto-70-1078

10.1128/AEM.60.1.56-63.1994

10.1128/MMBR.43.3.422-442.1979

10.1080/09583159309355278

Matar GM, 1993, Polymerase chain reaction‐based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from Rochalimaea species for subtyping, Journal of Clinical Microbiology, 31, 1730, 10.1128/jcm.31.7.1730-1734.1993

10.1099/00221287-107-2-319

Miller JC, 1990, Programs for analyzing restriction site or fragment data

Miller JH, 1972, Experiments in Molecular Genetics

10.1099/00207713-36-2-339

10.1007/BF00245277

10.1073/pnas.76.10.5269

Neilands JB, 1973, Inorganic Biochemistry, 167

10.1007/978-1-4757-0611-6_1

Palleroni NJ, 1984, Manual of Systematic bacteriology, 141

Palleroni N J., 1972, Deoxyribonucleic acid homologies among some Pseudomonas species, Journal of Bacteriology, 110, 1, 10.1128/jb.110.1.1-11.1972

10.1099/00207713-23-4-333

10.1099/00207713-23-2-111

10.1128/jb.175.4.973-981.1993

10.1128/jb.174.1.327-330.1992

Saitou N., 1987, The neighbor‐joining method: a new method for reconstructing phylogenetic trees, Molecular Biology Evolution, 4, 406

Seal SE, 1992, Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification, Applied and Environmental Microbiology, 58, 3759, 10.1128/aem.58.11.3759-3761.1992

10.1038/nbt1183-784

10.1099/00221287-43-2-159

Swofford DL, 1993, Paup: phylogenetic analysis using parsimony

10.1021/bi00525a025

10.1093/nar/19.24.6823

10.1128/jb.172.8.4238-4246.1990

10.1128/jb.173.2.697-703.1991

10.1146/annurev.py.26.090188.002115

10.1093/nar/18.24.7213

10.1093/nar/19.4.861

10.1111/j.1095-8312.1999.tb01918.x

10.1016/S0723-2020(84)80019-3

10.1016/S0723-2020(85)80007-2

10.1080/00288233.1978.10427397

10.1128/jb.173.7.2271-2277.1991