Fluorescent dyes for lymphocyte migration and proliferation studies
Tóm tắt
Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as being the most versatile in terms of long‐term tracking of lymphocytes and their ability to quantify lymphocyte proliferation. They are the intracellular covalent coupling dye carboxyfluorescein diacetate succinimidyl ester (CFSE) and the membrane inserting dye PKH26. Both dyes have the advantage that they can be used to track cell division, both
Từ khóa
Tài liệu tham khảo
Rannie GH, 1977, An experimental comparison of radioactive labels with potential application to lymphocyte migration studies in patients., Clin. Exp. Immunol., 29, 509
Ford WL, 1978, Handbook of Experimental Immunology, 23.1
Harp JA, 1990, Extravasation of lymphocytes via paracortical venules in sheep lymph nodes: Visualization using an intracellular fluorescent label., Vet. Immunol. Immunopathol., 31, 377
Maghazachi AA, 1990, Fate of intravenously administered rat lymphokine‐activated killer cells labeled with different markers., Cancer Immunol. Immunother., 24, 159
Brenan M, 1987, Simultaneous determination of viable and dead fluorescently labelled lymphocytes., J. Immunol. Meth., 102, 147, 10.1016/S0022-1759(87)80020-0
Weston SA, 1991, Modification of lymphocyte migration by mannans and phosphomannans: Different carbohydrate structures control entry of lymphocytes into spleen and lymph nodes., J. Immunol., 146, 4180, 10.4049/jimmunol.146.12.4180
Chiba K, 1998, FTY720, a novel immunosuppressant, induced sequestration of circulating mature lymphocytes by acceleration of lymphocyte homing in rats. I. FTY720 selectively decreases the number of circulating mature lymphocytes by acceleration of lymphocyte homing., J. Immunol., 160, 5037, 10.4049/jimmunol.160.10.5037
Renno T, 1999, Apoptosis of superantigen‐activated T cells occurs preferentially after a discrete number of cell divisions in vivo., J. Immunol., 162, 6312, 10.4049/jimmunol.162.11.6312
Khil LY, 1997, Insulin has a limited effect on the cell cycle progression in 3T3, L1 fibroblasts., Mol. Cells, 7, 742, 10.1016/S1016-8478(23)13373-5
Bradbury MG, 1996, The immunosuppressive compound 2‐acetyl‐4‐tetrahydroxybutyl imidazole inhibits the allogeneic MLR by sequestration of a recirculating subpopulation of T cells., Immunology, 87, 80
Dittel BN, 1999, Presentation of the self antigen myelin basic protein by dendritic cells leads to experimental autoimmune encephalomyelitis., J. Immunol., 163, 32, 10.4049/jimmunol.163.1.32
Slezak SE, 1989, Fluorescent in vivo tracking of hematopoietic cells. Part I. Technical considerations., Blood, 74, 2172, 10.1182/blood.V74.6.2172.2172
Boutonnat J, 1998, PKH26 probe in the study of the proliferation of chemoresistant leukemic sublines., Anticancer Res., 18, 4243
Hendrikx PJ, 1996, Homing of fluorescently labeled murine hematopoietic stem cells., Exp. Hematol., 24, 129
Wallace PK, 1993, Mechanisms of adoptive immunotherapy: Improved methods for in vivo tracking of tumor‐infiltrating lymphocytes and lymphokine‐activated killer cells., Cancer Res., 53, 2358