Fluorescence and Bioluminescence Genetically Encoded Probes for Imaging Protein Conformational Changes and Interactions in Living Cells and Animals

SLAS Technology - Tập 15 - Trang 396-404 - 2010
Muhammad Awais1
1Centre for Cell Imaging, School of Biological Sciences, University of Liverpool, Liverpool, UK

Tóm tắt

Rapid advancement in fluorescence and luminescence imaging technology is encouraging cell biologists to develop high-throughput assays for intracellular detection of bioactive small molecules, protein conformational changes, protein localization and dynamics, and measurement of protein—protein interactions in real time at the level of single living cells with high spatial and temporal resolution. In this report, we summarize the development of genetically encoded fluorescent and bioluminescent probes to study various molecular events in live cells and animals for potential drug candidates against different diseases.


Tài liệu tham khảo

10.1002/jcp.1030590302 10.1021/bi00056a003 10.1016/0378-1119(92)90691-H 10.1126/science.8303295 10.1073/pnas.91.26.12501 10.1038/13657 10.1074/jbc.C000338200 10.1038/nbt0102-87 10.1038/nbt1037 10.1371/journal.pone.0004391 10.1126/science.1168683 10.1038/nrm1741 10.1039/b913033k 10.1039/b907749a 10.1016/j.copbio.2008.06.003 10.1016/j.tibs.2007.08.003 10.1016/S0959-440X(00)00249-9 10.1074/jbc.272.20.13270 10.1038/42264 10.1016/S0896-6273(00)00056-8 Nikolaev V. O., 2004, J. Biol. Sci., 279, 37215 10.1021/ac0006167 10.1038/73767 10.1038/nbt0302-287 10.1074/jbc.M212167200 10.1073/pnas.0902150106 10.1039/b720034j 10.1038/nmeth.1235 10.1517/13543770902994397 10.1158/0008-5472.CAN-09-3206 10.1073/pnas.0909125106 10.1210/er.2003-0023 10.1038/nrd1551 10.2174/1568026033451736 10.1038/42750 10.1016/S0092-8674(00)81717-1 10.1016/S1097-2765(00)80424-4 10.1101/gad.13.24.3209 10.1038/415813a 10.1210/me.2003-0421 10.1073/pnas.122225699 10.1002/cbic.200700001 10.1002/anie.200503185 10.1021/ac030410g 10.1016/j.steroids.2007.08.006 10.1016/j.bios.2006.10.013 10.1006/taap.1996.8069 10.1210/endo.132.6.8504730 10.1289/ehp.961041296 10.1016/S0165-9936(97)00099-X 10.1016/S0960-0760(98)00027-2 10.1289/ehp.95103s7135 10.1006/rtph.1997.1149 10.1016/S0890-6238(97)00014-2 10.1124/mol.106.028134 10.1038/nrd2311 10.1016/j.copbio.2009.02.013 10.1016/j.aca.2005.06.026 10.1038/nmeth1030 10.1371/journal.pone.0005868 10.1002/anie.200700538 10.1073/pnas.0401722101 10.1073/pnas.0607385103 10.1021/ac061934u