Flexible Label-Free Quantitative Assay for Antibodies to Influenza Virus Hemagglutinins

American Society for Microbiology - Tập 17 Số 9 - Trang 1407-1416 - 2010
P.J. Carney1,2, Aleksandr S. Lipatov1,2, Arnold S. Monto1,2, Ruben O. Donis1,2, James Stevens1,2
1Influenza Division, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333
2University of Michigan School of Public Health, 109 Observatory Street, Ann Arbor, Michigan 48109

Tóm tắt

ABSTRACT During the initial pandemic influenza H1N1 virus outbreak, assays such as hemagglutination inhibition and microneutralization provided important information on the relative protection afforded by the population's cross-reactivity from prior infections and immunizations with seasonal vaccines. However, these assays continue to be limited in that they are difficult to automate for high throughput, such as in pandemic situations, as well as to standardize between labs. Thus, new technologies are being sought to improve standardization, reliability, and throughput by using chemically defined reagents rather than whole cells and virions. We now report the use of a cell-free and label-free flu antibody biosensor assay (f-AbBA) for influenza research and diagnostics that utilizes recombinant hemagglutinin (HA) in conjunction with label-free biolayer interferometry technology to measure biomolecular interactions between the HA and specific anti-HA antibodies or sialylated ligands. We evaluated f-AbBA to determine anti-HA antibody binding activity in serum or plasma to assess vaccine-induced humoral responses. This assay can reveal the impact of antigenic difference on antibody binding to HA and also measure binding to different subtypes of HA. We also show that the biosensor assay can measure the ability of HA to bind a model sialylated receptor-like ligand. f-AbBA could be used in global surveillance laboratories since preliminary tests on desiccated HA probes showed no loss of activity after >2 months in storage at room temperature, indicating that the same reagent lots could be used in different laboratories to minimize interlaboratory assay fluctuation. Future development of such reagents and similar technologies may offer a robust platform for future influenza surveillance activities.

Từ khóa


Tài liệu tham khảo

Abdiche, Y., D. Malashock, A. Pinkerton, and J. Pons. 2008. Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet. Anal. Biochem.377:209-217.

Beare, A. S., and R. G. Webster. 1991. Replication of avian influenza viruses in humans. Arch. Virol.119:37-42.

Beyer, W. E., A. M. Palache, J. C. de Jong, and A. D. Osterhaus. 2002. Cold-adapted live influenza vaccine versus inactivated vaccine: systemic vaccine reactions, local and systemic antibody response, and vaccine efficacy. A meta-analysis. Vaccine20:1340-1353.

Centers for Disease Control and Prevention. 2009. Serum cross-reactive antibody response to a novel influenza A (H1N1) virus after vaccination with seasonal influenza vaccine. MMWR Morb. Mortal. Wkly. Rep.58:521-524.

10.1128/jcm.24.1.157-160.1986

10.1128/jcm.23.1.66-72.1986

Concepcion, J., K. Witte, C. Wartchow, S. Choo, D. Yao, H. Persson, J. Wei, P. Li, B. Heidecker, W. Ma, R. Varma, L. S. Zhao, D. Perillat, G. Carricato, M. Recknor, K. Du, H. Ho, T. Ellis, J. Gamez, M. Howes, J. Phi-Wilson, S. Lockard, R. Zuk, and H. Tan. 2009. Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization. Comb. Chem. High Throughput Screen.12:791-800.

El-Madhun, A. S., R. J. Cox, and L. R. Haaheim. 1999. The effect of age and natural priming on the IgG and IgA subclass responses after parenteral influenza vaccination. J. Infect. Dis.180:1356-1360.

10.1128/JVI.79.5.2814-2822.2005

Gambaryan, A., R. Webster, and M. Matrosovich. 2002. Differences between influenza virus receptors on target cells of duck and chicken. Arch. Virol.147:1197-1208.

Gambaryan, A. S., and M. N. Matrosovich. 1992. A solid-phase enzyme-linked assay for influenza virus receptor-binding activity. J. Virol. Methods39:111-123.

10.1126/science.1176225

Hirst, G. K. 1942. The quantitative determination of influenza virus and antibodies by means of red cell agglutination. J. Exp. Med.75:47-64.

Hobson, D., R. L. Curry, A. S. Beare, and A. Ward-Gardner. 1972. The role of serum haemagglutination-inhibiting antibody in protection against challenge infection with influenza A2 and B viruses. J. Hyg. (Lond.)70:767-777.

Hocart, M. J., J. S. Mackenzie, and G. A. Stewart. 1990. Serum IgG subclass responses of humans to inactivated and live influenza A vaccines compared to natural infections with influenza A. J. Med. Virol.30:92-96.

Kayali, G., S. F. Setterquist, A. W. Capuano, K. P. Myers, J. S. Gill, and G. C. Gray. 2008. Testing human sera for antibodies against avian influenza viruses: horse RBC hemagglutination inhibition vs. microneutralization assays. J. Clin. Virol.43:73-78.

Knossow, M., and J. J. Skehel. 2006. Variation and infectivity neutralization in influenza. Immunology119:1-7.

Monto, A. S., S. E. Ohmit, J. G. Petrie, E. Johnson, R. Truscon, E. Teich, J. Rotthoff, M. Boulton, and J. C. Victor. 2009. Comparative efficacy of inactivated and live attenuated influenza vaccines. N. Engl. J. Med.361:1260-1267.

Ndifon, W., J. Dushoff, and S. A. Levin. 2009. On the use of hemagglutination-inhibition for influenza surveillance: surveillance data are predictive of influenza vaccine effectiveness. Vaccine27:2447-2452.

10.1056/NEJMoa0903810

10.1128/jvi.67.5.2552-2558.1993

Potter, C. W., and J. S. Oxford. 1979. Determinants of immunity to influenza infection in man. Br. Med. Bull.35:69-75.

Profeta, M. L., and G. Palladino. 1986. Serological evidence of human infections with avian influenza viruses. Brief report. Arch. Virol.90:355-360.

10.1128/JCM.37.4.937-943.1999

Russell, C. A., T. C. Jones, I. G. Barr, N. J. Cox, R. J. Garten, V. Gregory, I. D. Gust, A. W. Hampson, A. J. Hay, A. C. Hurt, J. C. de Jong, A. Kielso, A. I. Klimov, T. Kageyama, N. Komadina, A. S. Lapedes, Y. P. Lin, A. Mosterin, M. Obuchi, T. Odagiri, A. D. Osterhaus, G. F. Rimmelzwaan, M. W. Shaw, E. Skepner, K. Stohr, M. Tashiro, R. A. Fouchier, and D. J. Smith. 2008. Influenza vaccine strain selection and recent studies on the global migration of seasonal influenza viruses. Vaccine26(Suppl. 4):D31-D34.

Stephenson, I., R. G. Das, J. M. Wood, and J. M. Katz. 2007. Comparison of neutralising antibody assays for detection of antibody to influenza A/H3N2 viruses: an international collaborative study. Vaccine25:4056-4063.

Stephenson, I., A. Heath, D. Major, R. W. Newman, K. Hoschler, W. Junzi, J. M. Katz, J. P. Weir, M. C. Zambon, and J. M. Wood. 2009. Reproducibility of serologic assays for influenza virus A (H5N1). Emerg. Infect. Dis.15:1250-1259.

Stephenson, I., J. M. Wood, K. G. Nicholson, and M. C. Zambon. 2003. Sialic acid receptor specificity on erythrocytes affects detection of antibody to avian influenza haemagglutinin. J. Med. Virol.70:391-398.

Stevens, J., O. Blixt, L. M. Chen, R. O. Donis, J. C. Paulson, and I. A. Wilson. 2008. Recent avian H5N1 viruses exhibit increased propensity for acquiring human receptor specificity. J. Mol. Biol.381:1382-1394.

10.1016/j.jmb.2005.11.002

10.1126/science.1124513

Stevens, J., A. L. Corper, C. F. Basler, J. K. Taubenberger, P. Palese, and I. A. Wilson. 2004. Structure of the uncleaved human H1 hemagglutinin from the extinct 1918 influenza virus. Science303:1866-1870.

10.1038/nsmb.1566

Treanor, J. J., K. Kotloff, R. F. Betts, R. Belshe, F. Newman, D. Iacuzio, J. Wittes, and M. Bryant. 1999. Evaluation of trivalent, live, cold-adapted (CAIV-T) and inactivated (TIV) influenza vaccines in prevention of virus infection and illness following challenge of adults with wild-type influenza A (H1N1), A (H3N2), and B viruses. Vaccine18:899-906.

WHO manual on animal influenza diagnosis and surveillance. 2002

Zambon, M. 1998. Laboratory diagnosis of influenza, p. 291-313. In K. G. Nicholson, R. G. Webster, and A. J. Hay (ed.), Textbook of influenza. Blackwell, Oxford, United Kingdom.

Thông tin
Thông tin xuất bản

American Society for Microbiology

Tập 17 Số 9

1407-1416

Thông tin tác giả