Field‐based evidence for copper contamination induced changes of antibiotic resistance in agricultural soils

Wiley - Tập 18 Số 11 - Trang 3896-3909 - 2016
Hang‐Wei Hu1,2, Juntao Wang2, Jing Li2, Jun‐Jian Li1, Yibing Ma3, Deli Chen1, Ji‐Zheng He1,2
1Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
2State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China
3National Soil Fertility and Fertilizer Effects Long-term Monitoring Network, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Tóm tắt

Summary

Bacterial resistance to antibiotics and heavy metals are frequently linked, suggesting that exposure to heavy metals might select for bacterial assemblages conferring resistance to antibiotics. However, there is a lack of clear evidence for the heavy metal‐induced changes of antibiotic resistance in a long‐term basis. Here, we used high‐capacity quantitative PCR array to investigate the responses of a broad spectrum of antibiotic resistance genes (ARGs) to 4–5 year copper contamination (0–800 mg kg−1) in two contrasting agricultural soils. In total, 157 and 149 unique ARGs were detected in the red and fluvo‐aquic soil, respectively, with multidrug and β‐lactam as the most dominant ARG types. The highest diversity and abundance of ARGs were observed in medium copper concentrations (100–200 mg kg−1) of the red soil and in high copper concentrations (400–800 mg kg−1) of the fluvo‐aquic soil. The abundances of total ARGs and several ARG types had significantly positive correlations with mobile genetic elements (MGEs), suggesting mobility potential of ARGs in copper‐contaminated soils. Network analysis revealed significant co‐occurrence patterns between ARGs and microbial taxa, indicating strong associations between ARGs and bacterial communities. Structural equation models showed that the significant impacts of copper contamination on ARG patterns were mainly driven by changes in bacterial community compositions and MGEs. Our results provide field‐based evidence that long‐term Cu contamination significantly changed the diversity, abundance and mobility potential of environmental antibiotic resistance, and caution the un‐perceived risk of the ARG dissemination in heavy metal polluted environments.

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Tài liệu tham khảo

10.1093/femsec/fiv140

10.1046/j.1462-2920.2001.00161.x

10.3389/fmicb.2011.00158

10.1289/ehp.1206316

10.1016/j.tim.2006.02.006

Bastian M. Heymann S. andJacomy M.(2009) Gephi: an open source software for exploring and manipulating networks. In:International AAAI Conference on Weblogs and Social Media: San Jose California.

10.1038/ismej.2010.171

10.1038/nrmicro3439

10.1111/j.1472-765X.2004.01650.x

10.1021/es101798r

10.1080/10643380490434128

10.1086/595011

10.1038/nmeth.f.303

10.1093/bioinformatics/btp636

10.1016/S0964-8305(03)00044-1

10.2134/jeq2008.0128

10.1126/science.1120800

10.1038/nature10388

10.1128/AEM.03006-05

10.1093/bioinformatics/btq461

10.1016/j.pedobi.2015.03.002

10.1126/science.1220761

10.1038/nature13377

10.1038/ismej.2014.226

10.1038/nrmicro1204

10.1021/es102473z

10.1016/j.envpol.2015.12.033

10.1016/j.soilbio.2008.03.014

10.1016/j.mib.2011.04.009

10.1093/femsec/fiv169

10.1016/j.jhazmat.2012.07.040

10.1002/9780470253489

10.1371/journal.pone.0027300

10.1021/es011055j

10.1146/annurev.micro.55.1.709

10.1111/j.1574-6941.2012.01458.x

10.1038/ismej.2015.59

Li J., 2015, Field‐based evidence for consistent responses of bacterial communities to copper contamination in two contrasting agricultural soils, Front Microbiol, 6, 31

10.1073/pnas.1120238109

10.1128/CMR.00002-11

10.1128/AEM.01682-13

10.1128/AEM.66.9.3722-3726.2000

10.1016/j.cub.2014.03.036

10.1016/j.watres.2010.04.041

10.1021/es060413l

10.1021/es302657r

10.2134/jeq2008.0299

10.3389/fmicb.2012.00399

10.1111/1462-2920.12553

10.1021/es048468f

10.1111/j.1462-2920.2006.01091.x

10.1021/acs.est.5b01012

10.1128/AAC.37.4.825

10.1128/AEM.66.11.4605-4614.2000

10.1038/nprot.2008.73

10.1128/AEM.00062-07

10.1111/1462-2920.12578

10.1128/aem.63.11.4494-4503.1997

10.3389/fmicb.2014.00765

World Health Organization, 2014, Antimicrobial Resistance: Global Report on Surveillance

10.1111/j.1574-6941.2006.00154.x

10.1038/ismej.2008.8

10.1073/pnas.1222743110

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Wiley

Tập 18 Số 11

3896-3909

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