Fibroblastic response to treatment with different preparations rich in growth factors

Cell Proliferation - Tập 42 Số 2 - Trang 162-170 - 2009
Eduardo Anitua1, Mikel Sánchez2, Mar Zalduendo3, María de la Fuente3, Roberto Prado3, Gorka Orive3, Isabel Andı́a3
1Biotechnology Institute, BTI IMASD, Vitoria-Gasteiz, Spain.
2Unidad de Cirugía Artroscópica, Clínica USP‐La Esperanza, c/ La Esperanza 3, 01002 Vitoria‐Gasteiz, Spain
3Biotechnology Institute, BTI IMASD c/ Jose Ma Cajigal 19, 01007 Vitoria‐Gasteiz, Spain

Tóm tắt

AbstractObjectives:  Preparations rich in growth factors (PRGF) release them plus bioactive proteins at localized sites, with the aim of triggering healing and regenerative processes. The prevailing paradigm suggests that their influence on proliferation, angiogenesis and the extracellular matrix synthesis is minimal. However, variations in their composition and impact on different cell phenotypes have not been examined.Materials and methods:  Sixteen fibroblast cultures obtained from three different anatomical sites (skin, synovium and tendon) of 16 donors were exposed to the molecular pool released from PRGF scaffolds, with increasing amounts of platelets. We evaluated cell proliferation, secretion of angiogenic growth factors (VEGF and HGF), synthesis of type I collagen and hyaluronic acid (HA), considering platelet dose and anatomical origin of the cells. Activity of transforming growth factor‐beta (TGF‐β) in type I procollagen and HA synthesis was examined by adding exogenous TGF‐β to plasma preparations.Results:  All plasma preparations induced a significant proliferative response compared to non‐stimulated cells (P < 0.05). Maximum proliferation rate was obtained with PRGF with 2‐fold or 4‐fold platelet concentration. Exposure to PRGF stimulated VEGF synthesis exclusively in tendon cells (P < 0.05), which also exhibited a different pattern of HGF production (P < 0.05). PRGF enhanced HA synthesis (P < 0.05), but did not alter collagen I production. Platelet‐secreted TGF‐β may be involved in HA, but not in type I procollagen synthesis.Conclusions:  Optimizing composition and use of platelet‐rich products is crucial to enhancing the therapeutic potential of this technology. Our data show that the biological effects of PRGF may depend on concentration of platelets and on the anatomical source of the cells.

Từ khóa


Tài liệu tham khảo

10.1073/pnas.162488599

Werner S, 2003, Regulation of wound healing by growth factors and cytokines, Physiol. Rev, 83, 853, 10.1152/physrev.2003.83.3.835

10.4049/jimmunol.179.3.1595

10.1161/01.RES.0000252802.25497.b7

10.1161/01.CIR.0000033634.60453.22

10.1160/TH03-07-0440

10.1055/s-2004-833479

10.1016/j.tibtech.2006.02.010

Nurden AT, 2007, Platelets and wound healing, Front Biosci, 13, 3532

10.1016/j.biomaterials.2007.06.037

10.1016/j.tibs.2006.03.007

10.1111/j.1365-2133.2007.07914.x

10.1016/j.orthres.2004.08.015

10.1177/0363546506294078

10.1016/j.tips.2007.10.010

10.1111/j.1365-2184.2007.00430.x

10.1111/j.1600-0501.2005.01203.x

10.1210/en.139.2.559

10.1089/ten.2006.12.2707

10.1111/j.1524-475X.2007.00259.x

10.1002/jbm.b.30886

10.1016/S0092-8674(03)00432-X

10.1042/bst0270135

10.1016/j.addr.2007.08.015

10.1093/rheumatology/kem234

10.1002/jbm.a.30585

10.1097/01.prs.0000255543.43695.1d

10.1161/01.RES.0000155330.07887.EE

10.1111/j.1365-2249.2005.02824.x

10.1371/journal.pgen.0020119

10.1083/jcb.200608093

10.1016/j.cytogfr.2005.09.007

10.1038/labinvest.3700426

10.1073/pnas.1434429100

10.1038/nbt993

10.1083/jcb.200105058

Thông tin
Thông tin xuất bản

Cell Proliferation

Tập 42 Số 2

162-170

Thông tin tác giả