Fibroblast Colony Formation from Monolayer Cultures of Blood Cells

Transfusion - Tập 11 Số 6 - Trang 345-349 - 1971
E.A. Luria1,2, A. F. Panasyuk1,2, A.Y. Friedenstein1,2
1E. A. Luria, A. F. Panasyuk, and A. Y. Friedenstein, Immunomorphological Laboratory, The Gamaleya Institute of Epidemiology and Microbiology, USSR Academy of Medical Sciences, D-98, 2 Gameleya Street, Moscow, U.S.S.R.
2Immunomorphological Laboratory, The Gamaleya Institute of Epidemiology and Microbiology, USSR Academy of Medical Sciences, Moscow, U.SS.R.

Tóm tắt

The quantitative method of measuring fibroblast precursors in populations of hematopoietic cells demonstrates that their concentration in blood does not depend on the number of heart punctures during blood collection. This means that fibroblast colonies, originating in monolayer cultures of blood cells, develop from circulating blood cells. From the indices of 3H‐thymidine labeling it is calculated that the mean generation time for fibroblasts in seven and 11‐day cultures is 34 to 43 hours and that the proliferative pool in fibroblast colonies is not less than 84 and 79 per cent respectively. The concentration of fibroblast precursors among nucleated cells of peripheral blood of guinea pigs ranges between 0.2 and 2 × 10‐5.

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Tài liệu tham khảo

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Transfusion

Tập 11 Số 6

345-349

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