FIP200 regulates targeting of Atg16L1 to the isolation membrane

EMBO Reports - Tập 14 Số 3 - Trang 284-291 - 2013
Taki Nishimura1,2, Takeshi Kaizuka2, Ken Cadwell3,4, Mayurbhai H. Sahani2, Tatsuya Saitoh5,6, Shizuo Akira5,6, Herbert W. Virgin3, Noboru Mizushima1,2
1Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
2Department of Physiology and Cell Biology, Tokyo Medical and Dental University Tokyo 113-8519
3Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, Missouri 63110
4Skirball Institute and Department of Microbiology, New York University School of Medicine New York New York 10016 USA
5Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan
6Laboratory of Host Defense, WPI Immunology Frontier Research Center

Tóm tắt

Autophagosome formation is a dynamic process that is strictly controlled by autophagy‐related (Atg) proteins. However, how these Atg proteins are recruited to the autophagosome formation site or autophagic membranes remains poorly understood. Here, we found that FIP200, which is involved in proximal events, directly interacts with Atg16L1, one of the downstream Atg factors, in an Atg14‐ and phosphatidylinositol 3‐kinase‐independent manner. Atg16L1 deletion mutants, which lack the FIP200‐interacting domain, are defective in proper membrane targeting. Thus, FIP200 regulates not only early events but also late events of autophagosome formation through direct interaction with Atg16L1.

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10.1038/ncb1007-1102

10.1038/nrm2708

10.1111/j.1365-2443.2007.01050.x

10.4161/auto.6.6.12709

10.1242/jcs.094110

10.1091/mbc.e11-09-0746

10.4161/auto.6.4.11863

10.1016/j.devcel.2011.06.024

10.1091/mbc.e11-09-0785

10.1091/mbc.e07-12-1257

10.1091/mbc.e10-11-0893

10.1083/jcb.200712064

10.1091/mbc.e08-12-1248

10.4161/auto.5.7.9296

10.1016/j.ceb.2010.01.001

10.1016/j.febslet.2006.04.008

10.1242/jcs.00381

10.4161/auto.7.12.18025

10.1091/mbc.E07-12-1231

10.1038/nsmb.2475

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EMBO Reports

Tập 14 Số 3

284-291

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