Extranodal γδ‐T‐cell lymphoma in a dog with leishmaniasis

Veterinary Clinical Pathology - Tập 37 Số 3 - Trang 298-301 - 2008
Valentina Foglia Manzillo1, A. Pagano, Roberta Guglielmino, Luigi Gradoni, Brunella Restucci, Gætano Oliva
1Dipartimento di Scienze Cliniche Veterinarie, Facoltà di Medicina Veterinaria-Università di Napoli Federico II, Napoli, Italy.

Tóm tắt

Abstract: An 8‐year‐old intact male mongrel dog with alopecia and weight loss was referred to the Veterinary Faculty of Naples. The dog had pale mucous membranes, enlarged prescapular lymph nodes, and splenomegaly. Laboratory abnormalities included anemia, thrombocytopenia, and hyperglobulinemia. Bone marrow aspirate smears contained numerous Leishmania amastigotes and an immunofluorescent antibody titer was strongly positive (1:1280) for leishmaniasis. The dog was treated with a combination of meglumine antimoniate and allopurinol for 60 days and showed clinical improvement. Two months after the end of treatment the dog was again referred because of relapse of leishmaniasis and the presence of a firm subcutaneous mass on the medial right thigh. Based on cytologic examination of fine needle aspirates of the mass, a diagnosis of large‐cell lymphoma was made. Flow cytometry of tumor cells revealed γδ‐T‐cell lymphoma with a CD5+, CD3+, TCRγδ+, CD4−, CD8−, CD45RA+ immunophenotype. Using nested PCR, amastigotes were not detected in the neoplastic tissue. An association between leishmaniasis and hematopoietic tumors has been described rarely. γδ‐T cells may be involved in the host response to this parasite, and prolonged antigenic stimulation and chronic immunosuppression (typical of leishmaniasis) play a crucial role in the etiopathogenesis of T‐cell lymphoma.

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Tài liệu tham khảo

10.1136/vr.141.21.539

Evans DA., 1987, In Vitro Methods for Parasite Cultivation, 52

10.1128/JCM.44.4.1318-1322.2006

Lanotte G, 1979, Ecologie des leishmanioses dans le sud de France. Les formes evolutives de la leishmaniose viscerale canine. Ecology of leishmaniases in South France. [The evolutive forms of visceral canine leishmaniasis], Ann Parasitol Hum Comp, 54, 277

10.1111/j.1365-3024.1991.tb00550.x

Berrahal F, 1996, Canine leishmaniasis, identification of asymptomatic carriers by polymerase chain reaction and immunoblotting, 55, 273

MacEwen EG, 1996, Small Animal Clinical Oncology, 451

Moore PF, 2000, Schalm's Veterinary Hematology, 247

10.1016/S0165-2427(99)00051-3

10.1354/vp.37-6-637

10.1354/vp.40-5-556

Moore PF, 1998, Advances in Veterinary Dermatology, 77

Moore PF, 1994, Canine cutaneous epitheliotropic lymphoma (mycosis fungoides) is a proliferative disorder of CD8+ T‐cell, Am J Pathol, 144, 421

DeWolf‐Peeters C, 2000, γδ T‐cell lymphoma, a homogeneous entity?, 36, 294

Salhany KE, 1998, Subcutaneous panniculitis‐like‐ T‐cell lymphoma, clinicopathologic, immuno-phenotypic and genotypic analysis of alpha/beta and gamma/delta subtypes, 22, 881

10.1038/373255a0

10.1038/339544a0

Russo DM, 1993, Antigen‐reactive γδ T‐cells in human leishmaniasis, J Immunol, 151, 3712, 10.4049/jimmunol.151.7.3712

10.1016/0923-2494(91)90007-6

10.1016/S0065-308X(04)57001-X

Rosat JP, 1995, Expansion of γδ T‐cells in BALB/c mice infected with leishmania major is dependent upon Th2‐type CD4+ T cells, Infect Immun, 63, 3000, 10.1128/iai.63.8.3000-3004.1995

10.1136/vr.158.20.690

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Thông tin xuất bản

Veterinary Clinical Pathology

Tập 37 Số 3

298-301

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