Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Sự biểu hiện của các chỉ thị đặc trưng của tế bào hủy xương bởiPBMCs sau khi hợp nhất do PEG như một mô hình để nghiên cứu sự phân hóa tế bào hủy xương
Tóm tắt
Xương là một cơ quan hoạt động trao đổi chất tích cực, chịu ảnh hưởng của quá trình tái cấu trúc liên tục liên quan đến sự tiêu hủy bởi tế bào hủy xương và sự hình thành tiếp theo bởi tế bào tạo xương. Sự tham gia của tế bào hủy xương trong sự kiện sinh lý này được điều chỉnh bởi yếu tố kích thích thực bào (M-CSF) và ligand kích hoạt thụ thể của yếu tố nhân κB (RANKL). Sự hợp nhất của các tiền tế bào hủy xương đơn nhân là một sự kiện quan trọng cho sự phân hóa tế bào hủy xương và cho sự tiêu hủy xương. Ở đây, chúng tôi cho thấy rằng các tế bào máu ngoại biên có thể được hợp nhất thành công với polyethylen glycol (PEG) nhằm tạo ra các tế bào giống tế bào hủy xương sống có hoạt tính phosphatase B-axit kháng tartrate (TRAP) và hoạt động tiêu hủy xương. Các tế bào PBMC được hợp nhất bởi PEG biểu hiện các chỉ thị bổ sung tương thích với sự phân hóa osteoclastogenic như anhydrase carbonic II (CAII), thụ thể calcitonin (CR), cathepsin K (Cat K), tiểu đơn vị vacuolar ATPase (V-ATPase) C1 (V-ATPase), integrin β3, RANK và aminopeptidase N/CD13 bề mặt tế bào. Sự phân bổ lại actin trong các tế bào được hợp nhất bởi PEG bị ảnh hưởng bởi sự đồng bộ hóa chu kỳ tế bào tại các pha G0/G1 hoặc G2/M. Sự hợp nhất do PEG cũng dẫn đến sự biểu hiện của các kinase tyrosine c-Src và Syk ở trạng thái phosphoryl hóa. Hình ảnh từ kính hiển vi điện tử quét cho thấy các đặc điểm hình thái điển hình của các tế bào giống tế bào hủy xương. Các kết quả ở đây cho phép kết luận rằng sự hợp nhất PBMC do PEG gây ra cung cấp một hệ thống mô hình phù hợp cho việc hiểu các cơ chế tham gia vào sự hình thành tế bào hủy xương và để thử nghiệm những chiến lược điều trị mới.
Từ khóa
#hủy xương #tế bào hủy xương #PBMCs #PEG #phân hóa tế bào hủy xương #phosphatase B-axit kháng tartrateTài liệu tham khảo
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