Expression of miR‐486‐5p and its significance in lung squamous cell carcinoma

Journal of Cellular Biochemistry - Tập 120 Số 8 - Trang 13912-13923 - 2019
Sheng Yang1, Jing Sui1, Tong Liu1, Wenjuan Wu1, Siyi Xu1, Ying Zhang1, Yuepu Pu1, Xiaomei Zhang2, Yan Zhang2, Bo Shen2, Geyu Liang1
1Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University Nanjing Jiangsu P.R. China
2Department of Oncology Jiangsu Cancer Hospital Nanjing Jiangsu P.R. China

Tóm tắt

AbstractLung squamous cell carcinoma (LUSC) is one of the main histological types of lung cancer with high mortality. The role of microRNA‐486‐5p in LUSC remains unclear. In the current study, the aim was to explore miR‐486‐5p expression and its role in LUSC. The miR‐486‐5p expression was significantly low‐expressed in patients with LUSC from The Cancer Genome Atlas database, which was further confirmed in the Gene Expression Omnibus database, patients’ tissues, different cell lines by quantitative real‐time polymerase chain reaction, and the high‐throughput gene sequencing data of lung tissues of mice after a long‐term B(a)P exposure. The meta‐analysis was performed to evaluate the expression and diagnosis power of miR‐486‐5p (standard mean difference = −2.25; 95% confidence interval: −3.47 to −1.03; P = 0.0003; area under curve = 0.9082). Functional enrichment analysis revealed the potential function of miR‐486‐5p in LUSC using gene set enrichment analysis and clusterProfiler package in R software. At last, the hub genes (PTEN, TEK, PIK3R1, PPM1B, SMAD2, and SPTA1) of miR‐486‐5p were verified. In conclusion, miR‐486‐5p may be a LUSC antioncogene, playing an important role to serve as a biomarker in LUSC.

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Journal of Cellular Biochemistry

Tập 120 Số 8

13912-13923

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