Expression of miR-1, miR-133a, miR-133b and miR-206 increases during development of human skeletal muscle

BMC Developmental Biology - 2011
Andrie Koutsoulidou1, Nikolaos P. Mastroyiannopoulos1, Denis Furling2, James B. Uney3, Leonidas A. Phylactou1
1Department of Molecular Genetics, Function & Therapy, The Cyprus Institute of Neurology & Genetics, P.O. Box 2346, 1683, Nicosia, Cyprus
2UPMC Univ Paris 06, UM76, Institut de Myologie, and INSERM, U974 and CNRS, UMR7215, Paris, France
3The Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, Dorothy Hodgkin Building, University of Bristol, Whitson Street, Bristol, BS1 3NY, UK

Tóm tắt

Abstract Background MicroRNAs (miRNAs) are small RNA molecules that post-transcriptionally regulate gene expression and have been shown to play an important role during development. miR-1, miR-133a, miR-133b and miR-206 are expressed in muscle tissue and induced during muscle cell differentiation, a process that directs myoblasts to differentiate into mature myotubes, which are organized into myofibers. Although miR-1, miR-133a, miR-133b and miR-206 are well-studied in muscle, there is no information about their expression and function during human development. The purpose of this study was to determine the profile of these miRNAs in muscle cells isolated from different stages of human development. Results We examined the levels of miR-1, miR-133a, miR-133b and miR-206 during the development of human foetus. All four miRNA levels were found increased during late stages of human foetal muscle development. Increases in the expression levels of these miRNAs were proportional to the capacity of myoblasts to form myotubes. Changes in miRNA levels during human foetal development were accompanied by endogenous alterations in their known targets and also in their inducer, MyoD. Ectopic MyoD expression caused an induction of muscle cell differentiation in vitro, accompanied by an increase in the levels of miR-1, miR-133a, miR-133b and miR-206. Conclusions This study provides data about the profile of four miRNAs in human muscle cells isolated during different stages of foetal development. These results may shed light on the differentiation of muscle cells and regulation of muscle formation through miRNAs, during the development of human foetus.

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