Expression of coronin‐3 (coronin‐1C) in diffuse gliomas is related to malignancy

Journal of Pathology - Tập 214 Số 4 - Trang 415-424 - 2008
Dietmar Rudolf Thal1,2,3, C‐P Xavier1,4, André Rosentreter4, Stefan Linder5, B. Friedrichs6, Andreas Waha2, Torsten Pietsch2, Maria Stumpf4, Noegel Aa4,7, CS Clemen4,7
1Both authors contributed equally to this work and are listed in alphabetic order.
2Department of Neuropathology, University of Bonn Medical Centre, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany
3Institute of Pathology, Laboratory of Neuropathology, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany
4Centre for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, Germany
5Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Ludwig Maximilians-Universität, Pettenkoferstrasse 9, 80336 München, Germany
6Laboratory for Tumorgenetics, First Department of Internal Medicine, University Hospital Cologne, Kerpener Strasse 62, 50924 Cologne, Germany
7Centre for Molecular Medicine Cologne, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, Germany

Tóm tắt

AbstractCoronin‐3 (coronin‐1C), a homotrimeric F‐actin binding protein, has been shown to be important for cell migration and brain morphogenesis. Here, we present for the first time a detailed analysis of the expression pattern of coronin‐3 in human brain tumours and demonstrate that coronin‐3 expression correlates with malignant phenotype in diffuse gliomas. In general, the expression of coronin‐3 varies in different brain tumour entities. However, in diffuse gliomas, the number of coronin‐3 expressing tumour cells correlates with the degree of malignancy. High‐grade gliomas, such as anaplastic astrocytomas, anaplastic oligodendrogliomas, anaplastic oligoastrocytomas and glioblastomas, show high numbers of tumour cells positive for coronin‐3, while diffuse low‐grade gliomas, such as diffuse astrocytomas, oligodendrogliomas and oligoastrocytomas, exhibit low numbers of coronin‐3‐positive tumour cells. In order to explore and verify a contribution of coronin‐3 to the malignant phenotype of diffuse gliomas, we employed an efficient shRNA‐mediated coronin‐3 knockdown in U373 and A172 human glioblastoma cells. Coronin‐3 knockdown glioblastoma cells exhibited reduced levels of cell proliferation, cell motility and invasion into extracellular matrix compared to control cells. Together, our findings demonstrate evidence for a contribution of coronin‐3 expression in the malignant progression of diffuse gliomas. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

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Journal of Pathology

Tập 214 Số 4

415-424

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