Expression of Fas‐Fas Ligand in Murine Testis

American Journal of Reproductive Immunology - Tập 42 Số 6 - Trang 381-388 - 1999
Ji Xu1, Xiang Li2, Etsuko Mori2, Mao Wu Guo2, Ichiro Matsuda2, Hirohito Takaichi2, Takashi Amano3, Tsuneatsu Mori2
1Department of Immunology, Institute of Medical Science, University of Tokyo, Japan
2Department of Immunology, Institute of Medical Science, University of Tokyo, Tokyo Japan
3Laboratory of Animal Genetics and Breeding, Tokyo University of Agriculture, Tokyo, Japan

Tóm tắt

Xu JP, Li X, Mori E, Guo MW, Matsuda I, Takaichi H, Amano T, Mori T. Expression of Fas‐Fas ligand in murine testis. AJRI 1999; 42:381–388 © Munksgaard, CopenhagenPROBLEM: During spermatogenesis, it has been suggested that the number of germ cells to be matured is regulated and restricted through the apoptotic mechanism. In the present study, we investigated the expression and apoptotic role of Fas and Fas ligand (L) in the murine testis.METHOD OF STUDY: The expression of Fas‐FasL in the murine testis was assessed by reverse transcriptase‐polymerase chain reaction (RT‐PCR)‐Southern blot hybridization, in situ hybridization, and Western blot methods. The terminal deoxynucleotide transferase mediated dUTP‐nick end label (TUNEL) and DNA fragmentation methods were applied to detect the generation of apoptosis in germ cells.RESULTS: By means of RT‐PCR‐Southern blot hybridization, we demonstrated the positive expression of Fas in testicular germ cells, and of FasL in testicular cells, supporting the findings with in situ hybridization that Fas was localized in germ cells, whereas FasL was localized in Sertoli cells of murine testis. A specific band at 45 kDa was obtained in the lysates from testis and germ cells with Western blot analysis. Then, the co‐incubation of germ cells with Spodoptera frugiperda (Sf9)‐FasL cells in vitro resulted in the induction of apoptosis in germ cells detected by the TUNEL method. Furthermore, DNA fragmented ladders were also demonstrated in germ cells co‐incubated with Sf9‐FasL cells.CONCLUSION: Fas‐FasL system seemed to play an apoptotic role in spermatogenesis by the molecular interaction between FasL on Sertoli cells and Fas on germ cells.

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Tài liệu tham khảo

10.1002/ar.1091900410

Sinha Hikim AP, 1998, Spontaneous germ cell apoptosis in humans: evidence for ethnic differences in the susceptibility of germ cells to programmed cell death, J Clin Endocrinol Metab, 83, 152, 10.1210/jcem.83.1.4485

10.1210/me.7.5.643

10.1111/j.1365-2184.1992.tb01399.x

Bartke A., 1995, Apoptosis of male germ cells, a generalized or a cell type‐specific phenomenon, Endocrinology, 136, 3, 10.1210/endo.136.1.7828545

10.1210/endo.136.1.7828558

10.4049/jimmunol.148.4.1274

10.1084/jem.179.3.873

10.1083/jcb.133.2.335

10.1038/377630a0

10.1126/science.270.5239.1189

10.1126/science.275.5302.960

10.1126/science.2787530

10.1084/jem.169.5.1747

10.1016/0092-8674(91)90614-5

10.1038/364806a0

10.1016/0092-8674(93)90464-2

10.1016/0092-8674(93)90326-L

10.1006/bbrc.1994.2346

10.1111/j.1600-0897.1997.tb00249.x

10.1095/biolreprod52.2.279

10.1210/jc.81.7.2702

10.1159/000191295

Xu JP, 1997, Expression of Fas‐Fas ligand system associated with atresia in murine ovary, Zygote, 5, 321, 10.1017/S0967199400003907

10.1017/S096719949800032X

10.1210/endo.138.5.5110

10.1111/j.1365-2249.1991.tb05603.x

10.1016/0092-8674(94)90375-1

Hogan B., 1994, Manipulating the Mouse Embryo: A Laboratory Manual, 325

Luna LG, 1968, The Armed Forces Institute of Pathology, 140

10.1002/j.1939-4640.1987.tb02427.x

10.1007/BF00220527

10.1007/BF00217241

10.1210/endo-120-4-1615

10.1242/dev.122.6.1703

10.1093/emboj/16.9.2262

10.1038/ng0398-251

10.1210/en.136.11.5042

10.1038/380723a0

10.1016/0092-8674(95)90071-3

10.1016/S0092-8674(00)81266-0

10.1038/34112

10.1007/s002400050017

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American Journal of Reproductive Immunology

Tập 42 Số 6

381-388

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