Expression of FOXJ1 in hepatocellular carcinoma: Correlation with patients' prognosis and tumor cell proliferation

Molecular Carcinogenesis - Tập 52 Số 8 - Trang 647-659 - 2013
Hongwei Chen1,2, Xiaodong Huang2, Hong‐Chen Li1, Song He3, Runzhou Ni2, Cui‐Hua Chen2, Peng Chen2, Gang Wu2, Guihua Wang2, Yingying Wang2, Yunhong Zhao2, Yixin Zhang3, Aiguo Shen4,3,5, Huimin Wang4,5,2
1Clinical Laboratory, The First Hospital of Qinhuangdao, Hebei Province, P.R. China
2Medical Laboratory Center, Affiliated Hospital of Nantong University, Jiangsu Province, P.R. China
3Department of Pathology, Affiliated Cancer Hospital of Nantong University, Jiangsu Province, P.R. China
4Ai-Guo Shen, Department of Pathology, Affiliated Cancer Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu Province, P.R. China.
5Hui-Min Wang, Medical Laboratory Center, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu Province, P.R. China.

Tóm tắt

AbstractFOXJ1 is a member of the forkhead box (FOX) family of transcription factors. Recent studies suggested that FOXJ1 may function as a tumor suppressor gene in breast cancer. To investigate the potential roles of FOXJ1 in hepatocellular carcinoma (HCC), expression of FOXJ1 was first examined in eight paired frozen HCC and adjacent noncancerous liver tissues by Western blot, and we found that FOXJ1 was upregulated in HCC specimens. In addition, immunohistochemistry was performed to confirm our results in 108 HCC samples. Moreover, we also evaluated its relation with clinicopathological variables and the prognostic significance. The data showed that high expression of FOXJ1 was associated with histological grade (P < 0.001), and FOXJ1 was positively correlated with proliferation marker Ki‐67 (P < 0.01). Univariate analysis suggested that FOXJ1 expression was associated with poor prognosis (P < 0.001). Multivariate analysis indicated that tumor grade (P < 0.0001), metastasis (P = 0.0451), tumor size (P = 0.0459), FOXJ1 (P = 0.0011), and Ki‐67 (P = 0.0006) were independent prognostic markers for HCC. Furthermore, we noted that there existed the change of the level of FOXJ1 subcellular localization during cell‐cycle transition in HepG2 cells by immunofluorescence and cell fractionation. Besides, we employed FOXJ1 overexpression/knockdown approaches to investigate the effects of FOXJ1 on HCC cell proliferation and cell‐cycle distribution and found that overexpression of FOXJ1 can promote tumor cell proliferation and cell‐cycle transition. Our results suggested that FOXJ1 was overexpressed in HCCs and associated with histological grade and poor prognosis. Overexpression of FOXJ1 was also involved in tumor cell proliferation and cell‐cycle progression in HCC cell lines. © 2012 Wiley Periodicals, Inc.

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Molecular Carcinogenesis

Tập 52 Số 8

647-659

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