Expression Profile of Carotenoid Cleavage Dioxygenase Genes in Summer Squash (Cucurbita pepo L.)

Plant Foods for Human Nutrition - Tập 70 - Trang 200-206 - 2015
Clara I. González-Verdejo1, Ángeles Obrero1, Belén Román1, Pedro Gómez2
1IFAPA, Centro Alameda del Obispo, Área de Mejora y Biotecnología, Córdoba, Spain
2IFAPA Centro La Mojonera, Área de Mejora y Biotecnología, Almería, Spain

Tóm tắt

Carotenoids are important dietary components that can be found in vegetable crops. The accumulation of these compounds in fruit and vegetables is altered by the activity of carotenoid cleavage dioxygenases (CCDs) enzymes that produce their degradation. The aim of this work was to study the possible implication of CCD genes in preventing carotenoid storage in the horticultural crop summer squash (Cucurbita pepo L.). The relationship between the presence of these compounds and gene expression for CCDs was studied in three varieties showing different peel and flesh colour. Expression analysis for the CCD genes CpNCED1, CpNCED2, CpNCED3, CpNCED9, CpCCD1, CpCCD4a, CpCCD4b and CpCCD8 was carried out on different organs and at several fruit developmental stages. The results showed that the CpCCD4a and CpCCD4b genes were highly expressed in the variety with lowest carotenoid content suggesting a putative role in carotenoid accumulation pattern in summer squash fruit.

Tài liệu tham khảo

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