Expression Profile of Carotenoid Cleavage Dioxygenase Genes in Summer Squash (Cucurbita pepo L.)
Tóm tắt
Carotenoids are important dietary components that can be found in vegetable crops. The accumulation of these compounds in fruit and vegetables is altered by the activity of carotenoid cleavage dioxygenases (CCDs) enzymes that produce their degradation. The aim of this work was to study the possible implication of CCD genes in preventing carotenoid storage in the horticultural crop summer squash (Cucurbita pepo L.). The relationship between the presence of these compounds and gene expression for CCDs was studied in three varieties showing different peel and flesh colour. Expression analysis for the CCD genes CpNCED1, CpNCED2, CpNCED3, CpNCED9, CpCCD1, CpCCD4a, CpCCD4b and CpCCD8 was carried out on different organs and at several fruit developmental stages. The results showed that the CpCCD4a and CpCCD4b genes were highly expressed in the variety with lowest carotenoid content suggesting a putative role in carotenoid accumulation pattern in summer squash fruit.
Tài liệu tham khảo
Fraser PD, Bramley PM (2004) The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res 43:228–265
Krinsky NI, Johnson EJ (2005) Carotenoid actions and their relation to health and disease. Mol Aspects Med 26:459–516
Cazzonelli CI (2011) Carotenoids in nature: insights from plants and beyond. Funct Plant Biol 38:833–847
Rocha Ribeiro SM, Queiroz JH, Ribeiro L, de Queiroz ME, Campos FM, Pinheiro Sant’ana HM (2007) Antioxidant in mango (Mangifera indica L.) pulp. Plant Foods Hum Nutr 62:13–17
Rajyalakshmi P, Padmavathi TVM, Venkata Laxmi K (2003) Total carotenoid and beta carotene contents of other vegetables, roots and tubers, fruits and stored products collected from forest areas of Andhra Pradesh, South India. Plant Foods Hum Nutr 58:1–11
Obrero A, Gonzalez-Verdejo CI, Die JV, Gómez P, Del Río-Celestino M, Román B (2013) Carotenogenic gene expression and carotenoid accumulation in three varieties of Cucurbita pepo during fruit development. J Agric Food Chem 61:6393–6403
Lu S, Li L (2008) Carotenoid metabolism: biosynthesis, regulation and beyond. J Integr Plant Biol 50:778–785
Cazzonelli CI, Pogson BJ (2010) Source to sink: regulation of carotenoid biosynthesis in plants. Trends Plant Sci 15:266–274
Auldridge ME, McCarty DR, Klee HJ (2006) Plant carotenoid cleavage oxygenases and their apocarotenoid products. Curr Opin Plant Biol 9:315–321
Giuliano G, Al-Babili S, von Lintig J (2003) Carotenoid oxygenases: cleave it or leave it. Trends Plant Sci 8:145–149
Walter MH, Floss DS, Strack D (2010) Apocarotenoids: hormones, mycorrhizal metabolites and aroma volatiles. Planta 232:1–17
Tan BC, Joseph LM, Deng WT, Liu L, Li QB, Cline K, McCarty D (2003) Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family. Plant J 35:44–56
Kato M, Matsumoto H, Ikoma Y, Okuda H, Yano M (2006) The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit. J Exp Bot 57:2153–2164
Kato M, Matsumoto H, Ikoma Y, Kuniga T, Nakajima N, Yoshida T, Yano M (2007) Accumulation of carotenoids and expression of carotenoid biosynthetic genes and carotenoid cleavage dioxygenase genes during fruit maturation in the juice sacs of ‘Tamami’, ‘Kiyomi’ tangor, and ‘Wilking’ mandarin. J Japan Soc Hort Sci 76:103–111
Kang B, Zhao W, Hou Y, Tian P (2010) Expression of carotenogenic genes during the development and ripening of watermelon fruit. Sci Hort 124:368–375
Campbell R, Ducreux LJM, Morris WL, Morris JA, Suttle JC, Ramsay G, Bryan GJ, Hedley PE, Taylor MA (2010) The metabolic and developmental roles of carotenoid cleavage dioxygenase4 from potato. Plant Physiol 154:656–664
Brandi F, Bar E, Mourgues F, Horváth G, Turcsi E, Giuliano G, Liverani A, Tartarini S, Lewinsohn E, Rosati C (2011) Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism. BMC Plant Biol 11:24
Ma J, Li J, Zhao J, Zhou H, Ren F, Wang L, Gu C, Liao L, Han Y (2014) Inactivation of a gene encoding carotenoid cleavage dioxygenase (CCD4) leads to carotenoid-based yellow coloration of fruit flesh and leaf midvein in Peach. Plant Mol Biol Rep 32:246–257
Sun L, Yuan B, Zhang M, Wang L, Cui M, Wang Q, Leng P (2012) Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and b-carotene contents in tomato fruit. J Exp Bot 63:3097–3108
Blanca J, Canizares J, Roig C, Ziarsolo P, Nuez F, Picó B (2011) Transcriptome characterization and high throughput SSRs and SNPs discovery in Cucurbita pepo (Cucurbitaceae). BMC Genom 12:104
Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8:R19
Obrero A, Die JV, Roman B, Gomez P, Nadal S, González-Verdejo CI (2011) Selection of reference genes for gene expression studies in zucchini (Cucurbita pepo) using qPCR. J Agric Food Chem 59:5402–5411
Rubio A, Rambla JL, Santaella M, Gómez MD, Orzaez D, Granell A, Gómez-Gómez L (2008) Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both involved in β-ionone release. J Biol Chem 283:24816–24825
Simkin AJ, Underwood BA, Auldridge M, Loucas HM, Shibuya K, Schmelz E, Clark DG, Klee HJ (2004) Circadian regulation of the PhCCD1 carotenoid cleavage dioxygenase controls emission of β-ionone, a fragrance volatile of petunia flowers. Plant Physiol 136:3504–3514
Kohlen W, Charnikhova T, Lammers M, Pollina T, Tóth P, Haider I, Pozo MJ, de Maagd RA, Ruyter-Spira C, Bouwmeester HJ, López-Ráez JA (2012) The tomato CAROTENOID CLEAVAGE DIOXIGENASE8 (SlCCD8) regulates rhizosphere signaling, plant architecture and affects reproductive development through strigolactone biosynthesis. New Phytol 196:535–547
Simkin AJ, Schwartz SH, Auldridge M, Taylor MG, Klee HJ (2004) The tomato carotenoid cleavage dioxygenase 1 genes contribute to the formation of the flavor volatiles β-ionone, pseudoionone, and geranylacetone. Plant J 40:882–892
Bouvier F, Suire C, Muttere J, Camara B (2003) Oxidative remodeling of chromoplast carotenoids: identification of the carotenoid dioxygenase CsCCD and CsZCD genes involved in crocus secondary metabolite biogenesis. Plant Cell 15:47–62
Ohmiya A, Kishimoto S, Aida R, Yoshioka S, Sumitomo K (2006) Carotenoid cleavage dioxygenase (CmCCD4a) contributes to white color formation in chrysanthemum petals. Plant Physiol 142:1193–1201
Yoshioka S, Aida R, Yamamizo C, Shibata M, Ohmiya A (2012) The carotenoid cleavage dioxygenase 4 (CmCCD4a) gene family encodes a key regulator of petal color mutation in chrysanthemum. Euphytica 184:377–387