Exposure to endotoxin or other bacterial components might protect against the development of atopy

Clinical and Experimental Allergy - Tập 30 Số 9 - Trang 1230-1234 - 2000
Von Mutius1, Braun‐Fahrländer2, Schierl3, Riedler4, Ehlermann3, Maisch1, Waser2, Nowak3
1University Children's Hospital, Munich, Germany
2Institute of Social and Preventive Medicine, University of Basel, Switzerland
3Institute for Occupational and Environmental Medicine, University Munich, Germany
4Children's Hospital, Salzburg, Austria

Tóm tắt

Background

Several recent studies have shown that growing up on a farm confers significant protection against the development of atopy. These findings point particularly towards the importance of exposure to stable dust and farm animals. It has furthermore been reported that endotoxin, an intrinsic part of the outer membrane of gram negative bacteria, is abundant in environments where livestock and poultry is kept.

The aim of this study was therefore to measure the level of environmental endotoxin exposure in homes of farmers' children, children with regular contact to livestock and control children with no contact to farm animals.

Methods

Eighty‐four farming and nonfarming families were identified in rural areas in Southern Germany and Switzerland. Samples of settled and airborne dust were collected in stables, and of settled dust indoors from kitchen floors and the children's mattresses. Endotoxin concentrations were determined by a kinetic Limulus assay.

Results

Endotoxin concentrations were highest in stables of farming families, but were also significantly higher indoors in dust from kitchen floors (143 EU/mg vs 39 EU/mg, P < 0.001) and children's mattresses (49479 EU/m2 vs 9383 EU/m2, P < 0.001) as compared to control children from nonfarming families. In addition, endotoxin levels were also significantly higher in mattresses and dust from kitchen floors in households where children had regular contact to farm animals (38.6 EU/mg and 23340 EU/m2, respectively) as compared to control subjects.

Conclusion

We propose that the level of environmental exposure to endotoxin and other bacterial wall components is an important protective determinant for the development of atopic diseases in childhood.

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