Khám phá cơ chế phân tử của những biến đổi trong khớp ở bệnh hoại tử xương đầu xương đùi bằng cách sử dụng proteomics DIA và phân tích sinh học thông tin

Gang Zhao1, Yujie Liu1, Yue Zheng2, Mingyang An1, Zhilong Jia1, Junlin Zhang1, Zhongli Li1, Li Chunbao1
1Department of Orthopedics, the No.4 Medical Centre, Chinese PLA General Hospital, Beijing, 100048, China
2Department of Orthopaedics, Chinese PLA 984 Hospital, Beijing, 100029, China

Tóm tắt

Tóm tắt Mục đích Nghiên cứu này nhằm phân tích các protein quan trọng liên quan đến hoại tử xương đầu xương đùi (ONFH) và các tổn thương trong khớp của nó thông qua công nghệ proteomics lấy dữ liệu độc lập (DIA) và phân tích sinh học thông tin. Phương pháp Chúng tôi đã chọn ngẫu nhiên các mẫu từ những bệnh nhân ONFH đủ tiêu chuẩn và thu thập mẫu từ vùng hoại tử của đầu xương đùi và sụn chịu lực. Nhóm đối chứng bao gồm các mẫu từ cùng một vị trí ở những bệnh nhân có gãy cổ xương đùi. Với proteomics DIA, chúng tôi đã kiểm tra định lượng và định tính cả hai nhóm và phân tích các protein biểu hiện khác nhau (DEPs) giữa các nhóm. Ngoài ra, chúng tôi cũng đã làm giàu phân tích các chức năng của DEP bằng cách sử dụng các thuật ngữ trong gene ontology và các đường dẫn của Bách khoa toàn thư về Genes và Genomes Kyoto và xác thực các protein chính trong ONFH thông qua Western blot.

Từ khóa

#hoại tử xương #đầu xương đùi #proteomics #sinh học thông tin #protein chính

Tài liệu tham khảo

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