Exon 2 deletion splice variant of γ‐glutamyl carboxylase causes des‐γ‐carboxy prothrombin production in hepatocellular carcinoma cell lines

Molecular Oncology - Tập 2 - Trang 241-249 - 2008
Naoki Ueda1, Hidenori Shiraha1, Tatsuya Fujikawa1, Nobuyuki Takaoka1, Yutaka Nakanishi1, Mayumi Suzuki1, Noriyuki Matsuo1, Shigetomi Tanaka1, Shin-ichi Nishina1, Masayuki Uemura1, Akinobu Takaki1, Yasushi Shiratori1, Kazuhide Yamamoto1
1Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan

Tóm tắt

Using GGCX gene‐specific real‐time PCR, exon 2 deletion splice variant of vitamin K‐dependent γ‐glutamyl carboxylase (GGCX) mRNA was identified in HCC cell lines. Expressions of wild type and exon 2 deletion variant of GGCX were analyzed with relevance to DCP production in HCC cell lines. Hep3B, HepG2, HuH1, HuH7, and PLC/PRF/5 produced DCP, while SK‐Hep‐1, HLE, HLF, and JHH1 produced no detectable level of DCP. DCP‐producing cells expressed exon 2 deletion variant of GGCX mRNA and protein, while DCP‐negative cells expressed no detectable level of exon 2 deletion variant of GGCX. These results suggest that exon 2 deletion splice variant of GGCX causes dysfunction of GGCX enzyme activity resulting in DCP production in HCC cell lines.

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Molecular Oncology

Tập 2

241-249

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