Exogenous application of nitric oxide and spermidine reduces the negative effects of salt stress on tomato
Tóm tắt
Due to increasing soil salinity, the world agricultural output is being threatened by the shrinking area of fertile land. In the present study, we explored the interactive roles of nitric oxide (NO; 100 μM) and spermidine (SP; 200 μM) in ameliorating the effects of salt stress (NaCl; 100 mM) in tomato (Solanum lycopersicum L. var. Five Star) seedlings. NaCl stress reduced shoot and root length, shoot and root fresh weight, shoot and root dry weight plant -1 and leaf area leaf -1. NaCl stress also suppressed the biosynthesis of photosynthetic pigments (Chlorophyll a and b) and increased proline (Pro) content, membrane damage and lipid peroxidation by inducing reactive oxygen species (H2O2 and O2• - ) generation in roots and leaves, as well as electrolyte leakage (EL) and malondialdehyde (MDA) accumulation in leaves. However, applying NO and/or SP increased the activities of catalase, peroxidase, superoxide dismutase, glutathione reductase and ascorbate peroxidase and increased photosynthetic pigment (chlorophyll a and b) and Pro accumulation, as well as reducing H2O2 and O2• - and MDA content and EL, under salt stress. When tomato plants were treated with NO and SP simultaneously, NO signaling was further enhanced, which was confirmed by the addition of cPTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; NO scavenger].
Tài liệu tham khảo
Aebi H (1984) Catalase in vitro. Meth Enzymol 105:121–126
Arasimowicz-Jelonek M, Floryszak-Wieczorek J, KubiS J (2009) Interaction between polyamine and nitric oxide signaling in adaptive responses to drought in cucumber. J Plant Growth Regul 28:177–186
Barnes JD, Balaguer L, Manrique E, Elvira S, Davison AW (1992) A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environ Exp Bot 32:85–100
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free Proline for water-stress studies. Plant Soil 39:205–207
Beligni MV, Fath A, Bethke PC, Lamattina L, Jones RL (2002). Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone Layers 1. Plant Physiol 129:1642–1650
Bouchereau A, Aziz A, Larher F, Martin-Tanguy J (1999). Polyamines and environmental challenges: recent development. Plant Sci 140: 103–125
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248–254
Chance B, Maehly AC (1955). Assay of catalase and peroxidases. Meth Enzymol 2:764–775
FAO (2005) Global Network on Integrated Soil Management for Sustainable Use of Salt-affected Soils. Rome: FAO Land and Plant Nutrition Management Service. Available online at: http://www.fao.org/ag/agl/agll/spush
Fan HF, Du CX, Guo SR (2013) Nitric oxide enhances salt tolerance in cucumber seedlings by regulating free polyamine content. Environ Exp Bot 86:52–59
Fernández-Marcos M, Sanz L, Lorenzo Ó (2012) Nitric oxide: An emerging regulator of cell elongation during primary root growth. Plant Signal Behav 7:196–200
Filippou P, Antoniou C, Fotopoulos V (2013). The nitric oxide donor sodium nitroprusside regulates polyamine and proline metabolism in leaves of Medicago truncatula plants. Free Radic Biol Med 56:172–183
Foyer C, Halliwell B (1976). The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta 133:21–25
Fu JJ, Sun YF, Chu XT, Yang LY, Xu YF, Hu TM (2014). Exogenous nitric oxide alleviates shade-induced oxidative stress in tall fescue (Festuca arundinacea Schreb.). J Hortic Sci Biotechnol 89:193–200
Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59:309–314
Graziano M, Lamattina L (2005) Nitric oxide and iron in plants: an emerging and converging story. Trends Plant Sci 10:4–8
Groppa MD, Benavides MP (2008) Polyamines and abiotic stress: recent advances. Amino Acids 34:35–45
Gupta B, Huang B (2014). Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics 701596
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Hu L, Xiang L, Li S, Zou Z, Hu XH (2015) Beneficial role of spermidine in chlorophyll metabolism and D1 protein content in tomato seedlings under salinity-alkalinity stress. Physiol Plant 156: 468–477
Imai A, Matsuyama T, Hanzawa Y, Akiyama T, Tamaoki M, Saji H, Shirano Y, Kato T, Hayashi H, Shibata D, Tabata S, Komeda Y, Takahashi T (2004) Spermidine synthase genes are essential for survival of Arabidopsis. Plant Physiol 135:1565–1573
Khan MN, Siddiqui MH, Mohammad F, Naeem M (2012). Interactive role of nitric oxide and calcium chloride in enhancing tolerance to salt stress. Nitric Oxide 27:210–218
Kusano T, Berberich T, Tateda C, Takahashi Y (2008) Polyamines: essential factors for growth and survival. Planta 228:367–381
Li X, Gong B, Xu K (2014) Interaction of nitric oxide and polyamines involves antioxidants and physiological strategies against chillinginduced oxidative damage in Zingiber officinale Roscoe. Sci Hortic 170:237–248
Liu Y, Wu R, Wan Q, Xie G, Bi Y (2007) Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean roots. Plant Cell Physiol 48:511–522
Lutts S, Kinet JM, Bouharmont J (1995) Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. J Exp Bot 46:1843–1852
Mostofa MG, Fujita M (2013) Salicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems. Ecotoxicology 22:959–973
Munns R (2005) Genes and salt tolerance: bringing them together. New Phytol 167:645–663
Nahar K, Hasanuzzaman M, Alam MM, Rahman A, Suzuki T, Fujita M (2016). Polyamine and nitricoxide crosstalk: Antagonistic effects on cadmium Toxicity in mung bean plants through upregulating the metal detoxification, antioxidant defense and methylglyoxal detoxification systems. Ecotoxicol Environ Saf 126:245–255
Nakano G, Asada K (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidise in spinach chloroplasts. Plant Cell Physiol 22:867–880
Rodríguez-Serrano MA RÍA, Romero-Puertas MC, Zabalza ANA, Corpas FJ, Gómez M, Del Rio LA, Sandalio LM (2006) Cadmium effect on oxidative metabolism of pea (Pisum sativum L.) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo. Plant Cell Environ 29:1532–1544
Schopfer P, Plachy C, Frahry G (2001) Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol 125:1591–1602
Shu S, Guo SR, Yuan LY (2012) A review: polyamines and photosynthesis. In: Najafpour MM (ed) Advances in Photosynthesis-Fundamental Aspects. InTech, Rijeka, chapter 21, pp 439–464
Siddiqui MH, Al-Whaibi MH, Ali HM, Sakran AM, Basalah MO, Al Khaishany MYY (2013) Mitigation of nickel stress by the exogenous application of salicylic acid and nitric oxide in wheat. Aust J Crop Sci 7:1780–1788
Siddiqui MH, Al-Whaibi MH, Basalah MO (2011). Role of nitric oxide in tolerance of plants to abiotic stress. Protoplasma 248: 447–455
Siddiqui MH, Mohammad F, Khan MMA, Al-Whaibi MH (2012) Cumulative effect of nitrogen and sulphur on Brassica juncea L. genotypes under NaCl stress. Protoplasma 249:139–153
Siddiqui MH, Mohammad F, Khan MN (2009). Morphologicalandphysio biochemical characterization of Brassica juncea L. Czern.&Coss. genotypes under salt stress. J Plant Interact 4:67–80
Siddiqui MH, Mohammad F, Khan MN, Al-Whaibi MH, Bahkali AHA (2010) Nitrogen in relation to photosynthetic capacity and accumulation of osmoprotectant and nutrients in brassica genotypes grown under salt stress. Agric Sci China 9:71–680
Siddiqui MH, Alamri SA, Al-Khaishany MYY, Al-Qutami MA, Ali HM, Khan MN (2017). Sodium nitroprusside and indole acetic acid improve the tolerance of tomato plants to heat stress by protecting against DNA damage. J Plant Interact 12:177–186
Silveira V, Santa-Catarina C, Tun NN, Scherer GFE, Handro W, Guerra MP, Floh EIS (2006) Polyamine effects on the endogenous polyamine contents, nitric oxide release, growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia (Bert.) O. Ktze. Plant Sci 171:91–98
Thomas T, Thomas TJ (2001) Polyamines in cell growth and cell death: molecular mechanisms and therapeutic applications. Cell Mol Life Sci 58:244–258
Tun NN, Santa-Catarina C, Begum T, Silveira V, Handro W, Floh IS, Scherer FE (2006) Polyamines induce rapid biosynthesis of nitric oxide (NO) in Arabidopsis thaliana seedlings. Plant Cell Physiol 47:346–354
Verslues PE, Sharma S (2010). Proline metabolism and its implications for plant-environment interaction. Arabidopsis Book 8:e0140
Wang C, Zhang S, Wang P, Hou J, Qian J, Ao Y, Lu J, Li L (2011) Salicylic acid involved in the regulation of nutrient elements uptake and oxidative stress in Vallisneria natans (Lour.) Hara under Pb stress. Chemosphere 84:136–142
Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218:1–14
Wang Y, Luo Z, Mao L, Ying T (2016) Contribution of polyamines metabolism and GABA shunt to chilling tolerance induced by nitric oxide in cold-stored banana fruit. Food Chem 197:333–339
Wen XP, Ban Y, Pang XM, Moriguchi T (2011) Identification of differentially-expressed genes potentially related to stress tolerance in a transgenic line of European pear over-expressing an apple spermidine synthase gene (MdSPDS1). J Hortic Sci Biotechnol 86: 146–152
Wu XX, Zhu XH, Chen JL, Yang SJ, Ding HD, Zha DS (2013). Nitric oxide alleviates adverse salt-induced effects by improving the photosynthetic performance and increasing the anti-oxidant capacity of eggplant (Solanum melongena L.). J Hortic Sci Biotechnol 88: 352–360
Zhao H, Yang H (2008) Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd. Sci Hortic 116:442–447