Evolutionary origins of the emergent ST796 clone of vancomycin resistant<i>Enterococcus faecium</i>

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Andrew H. Buultjens1, Margaret M. C. Lam1, Susan A. Ballard2, Ian R. Monk1, Andrew A. Mahony3, Elizabeth A. Grabsch3, M. Lindsay Grayson3, Stanley Pang4,5, Geoffrey W. Coombs4,5, James O. Robinson6,5, Torsten Seemann7, Paul D. R. Johnson8,3, Benjamin P. Howden2, Timothy P. Stinear1
1Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia
2Microbiology Diagnostic Unit, Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Victoria, Australia
3Infectious Diseases Department, Austin Health, Heidelberg, Victoria, Australia
4Department of Microbiology, PathWest Laboratory Medicine-WA, Fiona Stanley Hospital, Murdoch, Western Australia, Australia
5School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia
6Department of Infectious Diseases, Fiona Stanley Hospital, Murdoch, Western Australia, Australia
7Victorian Life Sciences Computation Initiative, University of Melbourne, Carlton, Victoria, Australia
8Department of Medicine, University of Melbourne, Heidelberg, Victoria, Australia

Tóm tắt

From early 2012, a novel clone of vancomycin resistantEnterococcus faecium(assigned the multi locus sequence type ST796) was simultaneously isolated from geographically separate hospitals in south eastern Australia and New Zealand. Here we describe the complete genome sequence of Ef_aus0233, a representative ST796E. faeciumisolate. We used PacBio single molecule real-time sequencing to establish a high quality, fully assembled genome comprising a circular chromosome of 2,888,087 bp and five plasmids. Comparison of Ef_aus0233 to otherE. faeciumgenomes shows Ef_aus0233 is a member of the epidemic hospital-adapted lineage and has evolved from an ST555-like ancestral progenitor by the accumulation or modification of five mosaic plasmids and five putative prophage, acquisition of two cryptic genomic islands, accrued chromosomal single nucleotide polymorphisms and a 80 kb region of recombination, also gaining Tn1549and Tn916, transposons conferring resistance to vancomycin and tetracycline respectively. The genomic dissection of this new clone presented here underscores the propensity of the hospitalE. faeciumlineage to change, presumably in response to the specific conditions of hospital and healthcare environments.

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