Bằng chứng cho thấy tín hiệu plastid và ánh sáng hoạt động qua cùng một yếu tố cis-điều hòa trong các trình điều khiển của các gen nhân cho protein plastid

Springer Science and Business Media LLC - Tập 252 - Trang 631-639 - 1996
V. Kusnetsov1, C. Bolle1, T. Lübberstedt1, S. Sopory1, R. G. Herrmann1, R. Oelmüller1
1Botanisches Institut der Ludwig-Maximilians-Universität, Munich, Germany

Tóm tắt

Các gen mã hóa bởi nhân cho protein của hệ thống quang hợp đại diện cho một nhóm gen đặc biệt. Việc biểu hiện của chúng được kích thích hợp tác bởi các yếu tố khác nhau bao gồm giai đoạn phát triển của plastid và ánh sáng. Chúng tôi đã phân tích trong thuốc lá chuyển gen việc biểu hiện phụ thuộc vào plastid và ánh sáng của một loạt các loại trình điều khiển 5′ đã bị xóa của nhiều gen hạt cải bó xôi, các khối ghép của các đoạn trình điều khiển đã được xác định với trình điều khiển tối thiểu 35S RNA CaMV 90-bp, cũng như với các đột biến trong các trình tự có sự tương đồng với các yếu tố cis đã được xác định, để giải quyết câu hỏi liệu tín hiệu plastid và ánh sáng có hoạt động thông qua các yếu tố cis-điều hòa giống nhau hay khác nhau. Trong số 160 dòng chuyển gen khác nhau (đại diện cho 32 cấu trúc trình điều khiển từ bảy gen) đã được phân tích, không thể xác định được sự khác biệt đáng kể nào trong các phản ứng đối với hai con đường điều hòa này. Dữ liệu tương thích với ý tưởng rằng cả hai tín hiệu đều điều khiển việc biểu hiện của các gen nhân cho protein plastid thông qua cùng một yếu tố cis-điều hòa.

Từ khóa

#tín hiệu plastid #ánh sáng #gen mã hóa #protein plastid #các yếu tố cis-điều hòa

Tài liệu tham khảo

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