Evidence for solvent‐induced conformational changes of the soluble Dunaliella chloroplast coupling factor 1 (CF1)

FEBS Letters - Tập 171 - Trang 262-266 - 1984
Susanne Selman-Reimer1, Bruce R. Selman1
1Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison-WI 53706, USA

Tóm tắt

The ATPase activity of the chloroplast coupling factor 1 (CF1) isolated from the green alga Dunaliella is completely latent. A brief heat treatment irreversibly induces a Ca2+ ‐dependent activity. The Ca2+ dependent ATPase activity can be reversibly inhibited by ethanol, which changes the divalent cation dependency from Ca2+ to Mg2+. Both the Ca2+ ‐dependent and Mg2+ ‐dependent ATPase activities of heat‐treated Dunaliella CF1 are inhibited by monospecific antisera directed against Chlamydomonas reinhardi CF1. However, when assayed under identical conditions, the Ca2+ ‐dependent ATPase activity is significantly more sensitive to inhibition by the antisera than is the Mg2+ ‐dependent activity. These data are interpreted as indicating that soluble Dunaliella CF1 can exist in a variety of conformations, at least one of which catalyzes a Ca2+ ‐dependent ATPase and two or more of which catalyze an Mg2+ ‐dependent ATPase.

Tài liệu tham khảo

10.1016/0304-4173(76)90003-3 10.1016/B978-0-12-294301-0.50023-3 10.1016/S0021-9258(18)97376-X 10.1016/S0021-9258(18)99333-6 10.1021/bi00267a019 10.1021/bi00821a024 10.1016/S0021-9258(19)44721-2 10.1093/oxfordjournals.jbchem.a133473 10.1021/bi00522a020 Selman-Reimer S., 1984, Biochim. Biophys. Acta Finel M., 1984, Plant Physiol. 10.1104/pp.66.6.1196 10.1016/0005-2728(83)90041-5 10.1021/bi00646a002 10.1016/0005-2728(80)90227-3 10.1016/S0076-6879(80)70040-X
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Thông tin xuất bản

FEBS Letters

Tập 171

262-266

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