Evidence for Host-Bacterial Co-evolution via Genome Sequence Analysis of 480 Thai Mycobacterium tuberculosis Lineage 1 Isolates

Scientific Reports - Tập 8 Số 1
Prasit Palittapongarnpim1, Pravech Ajawatanawong1, Wasna Viratyosin2, Nat Smittipat2, Areeya Disratthakit3, Surakameth Mahasirimongkol3, Hideki Yanai4, Norio Yamada5, Supalert Nedsuwan6, Worarat Imasanguan6, Pacharee Kantipong6, Boonchai Chaiyasirinroje4, Jiraporn Wongyai4, Licht Toyo-oka7, Jody Phelan8, Julian Parkhill9, Taane G. Clark8, Martin L. Hibberd8, Wuthiwat Ruengchai1, Panawun Palittapongarnpim1, Tada Juthayothin2, Sissades Tongsima2, Katsushi Tokunaga7
1Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand
2National Centre for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Phahonyothin Road, Pathumthani, Thailand
3Department of Medical Sciences, Ministry of Public Health, Tiwanon Road, Nonthaburi, Thailand
4TB-HIV Research Foundation, Chiangrai, Thailand
5Research Institute of Tuberculosis, JATA, Kiyose, Japan
6Chiangrai Prachanukroh Hospital, Ministry of Public Health, Chiangrai, Thailand
7Department of Human Genetics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
8London School of Hygiene and Tropical Medicine, London, UK
9Welcome Trust Sanger Institute, Hinxton, Cambridge, UK

Tóm tắt

Abstract

Tuberculosis presents a global health challenge. Mycobacterium tuberculosis is divided into several lineages, each with a different geographical distribution. M. tuberculosis lineage 1 (L1) is common in the high-burden areas in East Africa and Southeast Asia. Although the founder effect contributes significantly to the phylogeographic profile, co-evolution between the host and M. tuberculosis may also play a role. Here, we reported the genomic analysis of 480 L1 isolates from patients in northern Thailand. The studied bacterial population was genetically diverse, allowing the identification of a total of 18 sublineages distributed into three major clades. The majority of isolates belonged to L1.1 followed by L1.2.1 and L1.2.2. Comparison of the single nucleotide variant (SNV) phylogenetic tree and the clades defined by spoligotyping revealed some monophyletic clades representing EAI2_MNL, EAI2_NTM and EAI6_BGD1 spoligotypes. Our work demonstrates that ambiguity in spoligotype assignment could be partially resolved if the entire DR region is investigated. Using the information to map L1 diversity across Southeast Asia highlighted differences in the dominant strain-types in each individual country, despite extensive interactions between populations over time. This finding supported the hypothesis that there is co-evolution between the bacteria and the host, and have implications for tuberculosis disease control.

Từ khóa


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