Evaluation of peroxyoxalate chemiluminescence postcolumn detection of fluphenazine in urine and blood plasma using high performance liquid chromatography

Biomedical Chromatography - Tập 5 Số 1 - Trang 47-52 - 1991
B. Mann1, Mary Lynn Grayeski1
1Department of Chemistry, Seton Hall University, South Orange, NJ 07079 USA

Tóm tắt

AbstractPeroxyoxalate chemiluminescence may be used for sensitive postcolumn detection of phenothiazine analytes separated by high performance liquid chromatography with appropriate optimization of measurement conditions such as solvent, pH and oxalate ester. Detectability of fluorescent analytes by chemical excitation varies greatly, but analytes with low oxidation potentials are generally more readily detected at low levels, as demonstrated for phenothiazines, an important class of fluorescent drugs. Some improvement in detection limits is observed for fluphenazine when chemiluminescence detection is compared to conventional fluorescence detection. Because of the specificity of chemical excitation, fewer interferences from fluorescent impurities in a urine matrix are observed.

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Biomedical Chromatography

Tập 5 Số 1

47-52

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